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甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白

Active Parathyroid Hormone Related Protein (PTHrP)

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

  • 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 產(chǎn)品包裝(模擬)
  • 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 產(chǎn)品包裝(模擬)
  • 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 Gene sequencing
  • APA819Mu01.jpg SDS-PAGE圖
  • 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 Figure. Western Blot; Sample: Recombinant PTHrP, Mouse.
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA819Mu01 甲狀旁腺激素相關(guān)蛋白(PTHrP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA819Mu02 甲狀旁腺激素相關(guān)蛋白(PTHrP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA819Mu01 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 Cell?culture;?Activity?Assays.
CPA819Mu21 甲狀旁腺激素相關(guān)蛋白(PTHrP)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA819Mu22 甲狀旁腺激素相關(guān)蛋白(PTHrP)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA819Mu31 甲狀旁腺激素相關(guān)蛋白(PTHrP)鑰孔血藍(lán)蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA819Mu01 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 WB; IHC; ICC; IP.
PAA819Mu02 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 WB; IHC; ICC; IP.
PAA819Mu08 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 WB; IHC; ICC; IP.
SEA819Mu 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA819Mu 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA819Mu 甲狀旁腺激素相關(guān)蛋白(PTHrP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA819Mu11 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Clinical and Experimental Metastasis Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133]
Stem Cells and Development Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004]
International journal of biological sciences PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447]
Investigational New Drugs Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814]
Toxicology and Applied Pharmacology Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094]
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060]
PLoS One 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665]
Endocrinology A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836]
Tumour Biol Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487]
35 Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628]
Bone Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610]
International Journal of Molecular Sciences A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911]
Journal of Orthopaedic Surgery and Research Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421]
International Immunopharmacology Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related?… []
Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни []
Cell Death Dis Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ?- [33510128]
Sci Rep Effects of whole-body vibration training in a cachectic C26 mouse model [34732809]
Acta Physiologica The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154]
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