多效生長因子(PTN)活性蛋白
Active Pleiotrophin (PTN)
HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule
- 編號APB309Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點9.6
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Pleiotrophin (PTN) is a 136 amino acid secreted heparin-binding cytokine that signals diverse functions, including lineage-specific differentiation of glial progenitor cells, neurite outgrowth, and angiogenesis. A considerable amount of research has been carried out to understand the mechanisms by which PTN regulates these events. PTN has now been shown to bind a diverse collection of receptors including Syndecan 1 (SDC1). A functional binding ELISA assay was conducted to detect the interaction of recombinant human PTN and recombinant human SDC1. Briefly, PTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SDC1-coated (2 ug/ml, 100 ul/well) microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with PTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human PTN and recombinant human SDC1 was shown in Figure 1, the EC50 was 3.47 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB309Hu01 | 多效生長因子(PTN)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB309Hu02 | 多效生長因子(PTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB309Hu61 | 多效生長因子(PTN)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB309Hu01 | 多效生長因子(PTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB309Hu61 | 多效生長因子(PTN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB309Hu06 | 多效生長因子(PTN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB309Hu02 | 多效生長因子(PTN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB309Hu01 | 多效生長因子(PTN)多克隆抗體 | WB |
| LAB309Hu81 | 多效生長因子(PTN)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB309Hu71 | 多效生長因子(PTN)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB309Hu22 | 多效生長因子(PTN)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB309Hu | 多效生長因子(PTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB309Hu | 多效生長因子(PTN)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB309Hu | 多效生長因子(PTN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Oncogene | Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophinMenin suppresses lung cancer through PTN [Nature: 2009273] |
| PLOS ONE | The Effects of Pleiotrophin in Proliferative Diabetic Retinopathy [Pubmed:25617851] |
| Infect Immun.? | Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice. [Pubmed:26755155] |
| In Vivo | Evaluation of Serum Biomarkers (FGF-2, HGF, MIF and PTN) in Patients With Testicular Germ Cell Cancer [Pubmed: 31662522] |
