表皮生長(zhǎng)因子受體2(ErbB2)活性蛋白
Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
CD340; EGFR2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; Epidermal Growth Factor Receptor 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
- 編號(hào)APB867Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) as known as HER2 (Human Epidermal Growth Factor Receptor 2), is a member of the EGFR family of receptor tyrosine kinases. It is a 185 kDa cell membrane receptor that plays a crucial role in cell signaling and proliferation.Besides,Plakophilin 2 (PKP2) has been identified as an interactor of ErbB2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ErbB2 and recombinant human PKP2. Briefly, ErbB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PKP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ErbB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ErbB2 and recombinant human PKP2 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB867Hu01 | 表皮生長(zhǎng)因子受體2(ErbB2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB867Hu02 | 表皮生長(zhǎng)因子受體2(ErbB2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB867Hu01 | 表皮生長(zhǎng)因子受體2(ErbB2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB867Hu02 | 表皮生長(zhǎng)因子受體2(ErbB2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB867Hu02 | 表皮生長(zhǎng)因子受體2(ErbB2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB867Hu01 | 表皮生長(zhǎng)因子受體2(ErbB2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB867Hu81 | 表皮生長(zhǎng)因子受體2(ErbB2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB867Hu71 | 表皮生長(zhǎng)因子受體2(ErbB2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB867Hu24 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB867Hu27 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB867Hu29 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB867Hu23 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB867Hu21 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB867Hu22 | 表皮生長(zhǎng)因子受體2(ErbB2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB867Hu | 表皮生長(zhǎng)因子受體2(ErbB2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB867Hu | 表皮生長(zhǎng)因子受體2(ErbB2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neuroscience Letter | Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrom [Cyagen: source] |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample [] |
