S100鈣結(jié)合蛋白B(S100B)活性蛋白
Active S100 Calcium Binding Protein B (S100B)
S100-B; NEF; S100Beta; S-100 protein subunit beta
- 編號(hào)APA567Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1440 ¥ 3600 ¥ 7200 ¥ 21600 ¥ 54000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Figure. The binding activity of S100B with RAGE.
Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA567Hu01 | S100鈣結(jié)合蛋白B(S100B)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA567Hu01 | S100鈣結(jié)合蛋白B(S100B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA567Hu01 | S100鈣結(jié)合蛋白B(S100B)多克隆抗體 | WB; IHC |
| LAA567Hu71 | S100鈣結(jié)合蛋白B(S100B)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA567Hu81 | S100鈣結(jié)合蛋白B(S100B)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA567Hu23 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA567Hu22 | S100鈣結(jié)合蛋白B(S100B)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA567Hu27 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA567Hu29 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA567Hu24 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA567Hu22 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA567Hu25 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA567Hu21 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA567Hu92 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體(辣根過(guò)氧化物酶標(biāo)記) | WB; IHC; ICC. |
| LAA567Hu82 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA567Hu72 | S100鈣結(jié)合蛋白B(S100B)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA567Hu | S100鈣結(jié)合蛋白B(S100B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA567Hu | S100鈣結(jié)合蛋白B(S100B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA567Hu | S100鈣結(jié)合蛋白B(S100B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA567Hu | 抗S100鈣結(jié)合蛋白B抗體(Anti-S100B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA567Hu | S100鈣結(jié)合蛋白B(S100B)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA567Hu | S100鈣結(jié)合蛋白B(S100B)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA567Hu01 | S100鈣結(jié)合蛋白B(S100B)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA567Hu01 | S100鈣結(jié)合蛋白B(S100B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
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