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性激素結(jié)合球蛋白(SHBG)活性蛋白

Active Sex Hormone Binding Globulin (SHBG)

ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin

編號APA396Hu61
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
性狀凍干粉
純度> 97%
等電點6.2
應(yīng)用Cell?culture;?Activity?Assays.
下載英文說明書中文說明書
規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Figure . Gene Sequencing (extract)

Figure. SDS-PAGE

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein, a molecule that consists of a carbohydrate plus a protein, and is produced mainly in your liver. It binds to three sex hormones found in both men and women: estrogen, dihydrotestosterone (DHT) and testosterone. Its job is to transport these hormones throughout your blood to other tissues in your body. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and bsa-conjugated testosterone(Testo) . Briefly, biotin-linked recombinant human SHBG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to bsa-conjugated Testo -coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of SHBG and Testo was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

聚丙烯酰胺凝膠電泳(PAGE)實驗服務(wù)

增值服務(wù)

BCA蛋白定量檢測試劑盒
 蛋白分子量MARKER
單克隆抗體定制服務(wù)
多克隆抗體定制服務(wù)
蛋白活性測定實驗服務(wù)
電泳遷移率變動分析(EMSA)實驗服務(wù)
緩沖液
 慢病毒包裝實驗服務(wù)
腺病毒包裝實驗服務(wù)
實時熒光定量PCR實驗服務(wù)
棘突蛋白受體結(jié)合結(jié)構(gòu)域
 蛋白G
蛋白 A

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA396Hu61	性激素結(jié)合球蛋白(SHBG)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu62	性激素結(jié)合球蛋白(SHBG)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu02	性激素結(jié)合球蛋白(SHBG)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA396Hu01	性激素結(jié)合球蛋白(SHBG)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPA396Hu63	性激素結(jié)合球蛋白(SHBG)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA396Hu61	性激素結(jié)合球蛋白(SHBG)活性蛋白	Cell?culture;?Activity?Assays.
APA396Hu62	性激素結(jié)合球蛋白(SHBG)活性蛋白	Cell?culture;?Activity?Assays.
PAA396Hu01	性激素結(jié)合球蛋白(SHBG)多克隆抗體	WB; IHC; ICC; IP.
PAA396Hu02	性激素結(jié)合球蛋白(SHBG)多克隆抗體	WB; IHC; ICC; IP.
LAA396Hu71	性激素結(jié)合球蛋白(SHBG)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
LAA396Hu81	性激素結(jié)合球蛋白(SHBG)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
MAA396Hu27	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu29	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu25	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB
MAA396Hu24	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu26	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu22	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu21	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
MAA396Hu23	性激素結(jié)合球蛋白(SHBG)單克隆抗體	WB; IHC; ICC; IP.
SEA396Hu	性激素結(jié)合球蛋白(SHBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA396Hu	性激素結(jié)合球蛋白(SHBG)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA396Hu01	性激素結(jié)合球蛋白(SHBG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志	參考文獻
Nutrition and Cancer	A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source]
international journal of environmental research and public health	Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886]
Journal of Life Science	Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric Syndrome [SMGHBM_2016_v26n9_1063]
Scientific Reports	Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
33	Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
Toxicology and?Applied?Pharmacology	Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats [pubmed:27746313]
中國醫(yī)藥導(dǎo)報	益腎祛濁方對PCOS-IR大鼠Ghrelin、SHBG及VF的影響 [yycyzx201733003]
Journal of Endocrinology	Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
Biomed Pharmacother	Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis [34029952]
Food Science and Biotechnology	Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats [33868755]
Nutrients	Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]

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