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Slit同源物2(Slit2)活性蛋白

Active Slit Homolog 2 (Slit2)

SLIL3

  • Slit同源物2(Slit2)活性蛋白 產品包裝(模擬)
  • Slit同源物2(Slit2)活性蛋白 產品包裝(模擬)
  • APA672Ra01.png Figure. SDS-PAGE
  • Slit同源物2(Slit2)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Figure. The binding activity of Slit2 with GREM1.
Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。

相關產品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
APA672Ra01 Slit同源物2(Slit2)活性蛋白 Cell?culture;?Activity?Assays.
RPA672Ra01 Slit同源物2(Slit2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA672Ra01 Slit同源物2(Slit2)多克隆抗體 WB; IHC; ICC; IP.
LAA672Ra71 Slit同源物2(Slit2)多克隆抗體(生物素標記) WB; IHC; ICC.
MAA672Ra21 Slit同源物2(Slit2)單克隆抗體 WB; IHC; ICC; IP.
SEA672Ra Slit同源物2(Slit2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA672Ra Slit同源物2(Slit2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Cancer Research SLIT2 Attenuation during Lung Cancer Progression Deregulates β-Catenin and E-Cadherin and Associates with Poor Prognosis [AACR: 702543]
Carcinogenesis Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line [OxfordJournals: source]
PLoS?One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
ESC Heart Fail Crosstalk between the activated Slit2¨CRobo1 pathway and TGF?\|?1 signalling promotes cardiac fibrosis [33236535]
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