轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)活性蛋白
Active Transforming Growth Factor Beta 2 (TGFb2)
TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
- 編號(hào)APA218Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1980 ¥ 4950 ¥ 9900 ¥ 29700 ¥ 74250
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Transforming growth factor-beta 2 (TGF-β2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Besides, Vitronectin (VTN) has been identified as an interactor of TGF-β2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGF-β2 and recombinant rat VTN. Briefly, TGF-β2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to VTN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TGF-β2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-β2 and VTN was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TGF-β2 with VTN.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA218Ra01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA218Ra02 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA218Ra03 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA218Ra04 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA218Ra01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA218Ra01 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA218Ra21 | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA218Ra | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA218Ra | 轉(zhuǎn)化生長(zhǎng)因子β2(TGFb2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| International Journal of Chemical Studies | Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528] |
| JCI Insight | Angiocrine signals regulate quiescence and therapy resistance in bone metastasis [Pubmed: 31292293] |
| Mol Med | Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma [34362313] |
| Food Chem | Effect of convection and microwave heating on the retention of bioactive components in human milk [34896952] |
