VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)活性蛋白
Active VGF Nerve Growth Factor Inducible (VGF)
NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide
- 編號(hào)APB166Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)4.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 2232 ¥ 5580 ¥ 11160 ¥ 33480 ¥ 83700
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
SDS-PAGE圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. Besides, Early Growth Response Protein 1 (EGR1) has been identified as an interactor of VGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse VGF and recombinant mouse EGR1. Briefly, EGR1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to VGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti- EGR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630nm immediately. The binding activity of VGF and EGR1 was shown in Figure 1, the ED50?for this effect is 0.515 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPB166Mu61 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB166Mu02 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB166Mu01 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB166Mu61 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB166Mu01 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB166Mu01 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB166Mu02 | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)多克隆抗體 | WB; IHC; ICC; IP. |
| SEB166Mu | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB166Mu | VGF神經(jīng)生長因子誘導(dǎo)蛋白(VGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Neuroendocrinology | Proteomic analysis of cerebrospinal fluid from obese women with idiopathic intracranial hypertension: a new approach for identifying new candidates in the pathogenesis of obesity [PubMed: 22296024] |
| J Psychiatr Res | Low VGF is associated with executive dysfunction in patients with major depressive disorder [Pubmed:35738161] |
