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載脂蛋白B(APOB)單克隆抗體

Monoclonal Antibody to Apolipoprotein B (APOB)

Apo-B; FLDB; Apo B-100; Apo B-48

編號MAC003Hu22
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)C3
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 NPC003Hu01-載脂蛋白B(APOB)天然蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/ml
且適物種Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 634 1478 ¥ 1478 ¥ 2112 ¥ 5280 ¥ 21120
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

IHC圖
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 30μg/ml Mouse Anti-Human APOB Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Figure. Western Blot; Sample: Recombinant APOB, Human.
Western Blot; Sample: Human Serum;
Primary Ab: 3μg/ml Mouse Anti-Human APOB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Human Hepa1-6 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human APOB Monoclonal Antibody (Catalog MAC003Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對APOB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別APOB。

用法

Western blotting: 0.5-3μg/mL
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(xué)(IHC)實驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實驗服務(wù)
流式細(xì)胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實時熒光定量PCR實驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
NPC003Hu01	載脂蛋白B(APOB)天然蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu02	載脂蛋白B(APOB)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu01	載脂蛋白B(APOB)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAC003Hu02	載脂蛋白B(APOB)多克隆抗體	WB; IHC; ICC; IP.
PAC003Hu01	載脂蛋白B(APOB)多克隆抗體	WB; IHC; ICC; IP.
LAC003Hu81	載脂蛋白B(APOB)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
LAC003Hu71	載脂蛋白B(APOB)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAC003Hu24	載脂蛋白B(APOB)單克隆抗體	WB; IHC; ICC; IP.
MAC003Hu25	載脂蛋白B(APOB)單克隆抗體	WB; IHC; ICC; IP.
MAC003Hu23	載脂蛋白B(APOB)單克隆抗體	WB; IHC; ICC; IP.
MAC003Hu22	載脂蛋白B(APOB)單克隆抗體	WB
MAC003Hu21	載脂蛋白B(APOB)單克隆抗體	WB
MAC003Hu26	載脂蛋白B(APOB)單克隆抗體	WB; IHC; ICC; IP.
FAC003Hu06	抗載脂蛋白B(APOB)單克隆抗體	Flow cytometry.
FAC003Hu04	抗載脂蛋白B(APOB)單克隆抗體	Flow cytometry.
FAC003Hu02	抗載脂蛋白B(APOB)單克隆抗體	Flow cytometry.
FAC003Hu08	抗載脂蛋白B(APOB)單克隆抗體	Flow cytometry.
SEC003Hu	載脂蛋白B(APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEC003Hu	載脂蛋白B(APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEC003Hu	抗載脂蛋白B抗體(Anti-APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antibody Detection.
SCC003Hu	載脂蛋白B(APOB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMC003Hu	載脂蛋白B(APOB)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Journal of Lipid Research	Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice [Jlr: M019463]
J Clin Endocrinol Metab.?	Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia [Pubmed:25781360]
Biochim Biophys Acta	Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Am J Cardiovasc Dis	Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice [PubMed: 26064792]
The Journal of Biological Chemistry	Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation [Pubmed:27284008]
United States Patent Application	COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES [y2016:0152988.html]
alcohol and alcoholism	Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. [pubmed:27659607]
Frontiers in Physiology	Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats [pubmed:28798692]
Food?Research International	Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541]
Journal of The Science of Food and Agriculture	Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773]
European?Journal of Pharmacology	The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981]
Nature Communications	Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794]
	Study of in Vivo Serum Lipid Regulation with Ulmus macrocarpa Hance Extract in Rats []
Nat Commun	GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]

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