胱天蛋白酶1(CASP1)多克隆抗體
Polyclonal Antibody to Caspase 1 (CASP1)
ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase
- 編號(hào)PAB592Po01
- 物種Sus scrofa; Porcine (Pig，豬) 相同的名稱(chēng)，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類(lèi)型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB592Po01-胱天蛋白酶1(CASP1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 720 ¥ 1680 ¥ 2400 ¥ 6000 ¥ 24000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Porcine Cardiac Muscle Tissue; Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Porcine Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Porcine Spleen Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Porcine Pancreas Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Porcine Small intestine Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;Sample: Porcine Small intestine TissuePrimary Ab: 10μg/ml Rabbit Anti-Porcine CASP1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)
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FITC staining on IF;
Sample: Human U2OS cell;
Primary Ab: 30μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;<br/>Sample: U2OS cell<br/>Primary Ab: 30μg/ml Rabbit Anti-Porcine CASP1 Antibody<br/>Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CASP1, Porcine.
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Western Blot; Sample: Lane1: Porcine Spleen lysate; Lane2: Porcine Lung lysate; Lane3: Raji cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type Raji cell lysate; <br/>Lane 2: CASP1 knockout Raji cell lysate; <br/>Predicted MW: 10,30,35,43,45kDa <br/>Observed MW: 42kDa<br/>Primary Ab: 5μg/ml Rabbit Anti-Porcine CASP1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)CASP1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CASP1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB592Po01 | 胱天蛋白酶1(CASP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB592Po01 | 胱天蛋白酶1(CASP1)多克隆抗體 | WB,IHC,ICC/IF |
| LAB592Po71 | 胱天蛋白酶1(CASP1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434] |
| Fertil Steril | Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” [PubMed: 26474737] |
| PLoS One | Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response. [pubmed:28704412] |
| Journal of?Virology | NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination [Pubmed:29386291] |
| Journal of Translational Medicine | Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? [Pubmed:29665864] |
| Reproductive?Sciences | A role for the inflammasome in spontaneous labor at term [Pubmed:27852921] |
| Journal?of?Virology | CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSES [Pubmed: 29386291] |
| ACTA?Otorhinolaryngologica Italica | NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media [Pubmed: 30676848] |
| American Journal of Reproductive Immunology | Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259] |
| Taiwanese Journal of Obstetrics & Gynecology | Serum Caspase-1 levels in women with polycystic ovary syndrome [Pubmed: 32127139] |
| Iranian Journal of Allergy, Asthma and Immunology | Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients [] |
| ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES [] | |
| Norwegian Journal of development of the International Science | CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER [] |
| LIFE SCIENCES | The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation [34560082] |
| Biol Reprod | The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice [34632484] |
| J Mol Neurosci | TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion Injury [Pubmed:35041191] |
| Viruses | Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion [Pubmed:35062292] |
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