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彈性蛋白(ELN)多克隆抗體

Polyclonal Antibody to Elastin (ELN)

WBS, WS, SVAS; Tropoelastin; Supravalvular Aortic Stenosis; Williams-Beuren Syndrome

編號PAB337Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB337Hu01-彈性蛋白(ELN)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度0.42mg/ml
且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colon Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Sample: Human Spleen Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant ELN, Human.
Western Blot; Sample: Human Lung lysate
Primary Ab: 1μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Skin lysate
Primary Ab: 2μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對ELN的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別ELN。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB337Hu01	彈性蛋白(ELN)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPB337Hu61	彈性蛋白(ELN)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
CPB337Hu21	彈性蛋白(ELN)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
CPB337Hu31	彈性蛋白(ELN)鑰孔血藍(lán)蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
PAB337Hu01	彈性蛋白(ELN)多克隆抗體	WB; IHC; ICC; IP.
PAB337Hu08	彈性蛋白(ELN)多克隆抗體	WB; IHC; ICC; IP.
LAB337Hu81	彈性蛋白(ELN)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
LAB337Hu71	彈性蛋白(ELN)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAB337Hu21	彈性蛋白(ELN)單克隆抗體	WB
MAB337Hu22	彈性蛋白(ELN)單克隆抗體	WB; IHC; ICC; IP.
MAB337Hu29	彈性蛋白(ELN)單克隆抗體	WB; IHC; ICC; IP.
MAB337Hu24	彈性蛋白(ELN)單克隆抗體	WB; IHC; ICC; IP.
MAB337Hu23	彈性蛋白(ELN)單克隆抗體	WB; IHC.
MAB337Hu25	彈性蛋白(ELN)單克隆抗體	WB; IHC; ICC; IP.
MAB337Hu27	彈性蛋白(ELN)單克隆抗體	WB; IHC; ICC; IP.
HEB337Hu	彈性蛋白(ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
SEB337Hu	彈性蛋白(ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEB337Hu	抗彈性蛋白抗體(Anti-ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antibody Detection.
SCB337Hu	彈性蛋白(ELN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
KSB337Hu01	彈性蛋白(ELN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Clinical & Experimental Immunology	Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies [PubMed: 22288597]
Stem Cell Research & Therapy	Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658]
Journal of Photochemistry and Photobiology B: Biology	A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262]
PLoS One.	Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Nutrition & Diabetes	Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling. [pubmed:27941938]
FREE RADICAL BIOLOGY AND MEDICINE	Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts [33482334]
	GENE THERAPY DNA VECTOR VTvaf17, METHOD OF PRODUCTION; ESCHERICHIA COLI STRAIN SCS110-AF, METHOD OF PRODUCTION; ESCHERICHIA??- []
ARCHIV?EUROMEDICA	CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE []

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