成纖維細(xì)胞生長(zhǎng)因子21(FGF21)多克隆抗體
Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21)
- 編號(hào)PAC918Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 -
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 450 ¥ 1050 ¥ 1500 ¥ 3750 ¥ 15000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Pancreas Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human FGF21 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human FGF21 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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WB圖
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293F line transfected with human FGF21 (Transient transfection) was stained with rabbit Anti-human FGF21 Polyclonal Antibody (Catalog # PAC918Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Chos line transfected with human FGF21 (Transient transfection) was stained with rabbit Anti-human FGF21 Polyclonal Antibody (Catalog # PAC918Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)FGF21的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別FGF21。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學(xué)：5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué)：5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC918Hu01 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC918Hu61 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPC918Hu61 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC918Hu01 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAC918Hu01 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)多克隆抗體 | WB; IHC; ICC; IP. |
| FAC918Hu01 | 抗成纖維細(xì)胞生長(zhǎng)因子21(FGF21)多克隆抗體 | Flow cytometry. |
| MAC918Hu21 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC918Hu23 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC918Hu24 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC918Hu22 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC918Hu25 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC918Hu27 | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC918Hu | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEC918Hu | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC918Hu | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| CCC918Hu | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC918Hu | 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Lipids in Health and Disease | Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs [Lipidworld: source] |
| Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression [Endocrine:Source] |
| Biochemical and Biophysical Research Communications | Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH [Pubmed:25449271] |
| Free Radic Biol Med | Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway [PubMed: 25843656] |
| Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484] |
| American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839] |
| Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
| Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим?… [] | |
| Physiol Res | Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. [Pubmed: 32469231] |
| Chinese Journal of Osteoporosis | 2 型糖尿病患者血清FGF21, 骨連結(jié)蛋白和骨轉(zhuǎn)換指標(biāo)的相關(guān)性研究. [] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose [] |
| Medicine (Baltimore) | Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort [34918690] |
| Annals of Agricultural and Environmental Medicine | Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis [34969228] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074] |
| Lab Invest | Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury [Pubmed:35488034] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients [] |
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