谷氨酰胺酶(GLS)多克隆抗體
Polyclonal Antibody to Glutaminase (GLS)
GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase
- 編號PAJ026Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPJ026Mu01-谷氨酰胺酶(GLS)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GLS, Mouse.
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Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: GLS knockout 293T cell lysate;
Predicted MW: 73,65kd
Observed MW: 60kd
Primary Ab: 2μg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對GLS的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GLS。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ026Mu01 | 谷氨酰胺酶(GLS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ026Mu02 | 谷氨酰胺酶(GLS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ026Mu02 | 谷氨酰胺酶(GLS)多克隆抗體 | WB; IHC; ICC; IP. |
| PAJ026Mu01 | 谷氨酰胺酶(GLS)多克隆抗體 | WB; IHC |
| SEJ026Mu | 谷氨酰胺酶(GLS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ026Mu | 谷氨酰胺酶(GLS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
| Journal of?Affective?Disorders | Glutamatergic hypofunction in medication-free major depression: Secondary effects of affective diagnosis and relationship to peripheral glutaminase [Pubmed:29544167] |
| ?？?/td> | King's Research Portal [Doi: 10.1016/j.jad.2018.02.059] |
| bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers [Pubmed: 32698888] |
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