谷胱甘肽過氧化酶4(GPX4)多克隆抗體
Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4)
MCSP; PHGPx; SnGPx; SnPHGPx; Phospholipid Hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
- 編號PAC994Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC994Hu01-谷胱甘肽過氧化酶4(GPX4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human GPX4 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;<br/>Samples: Human Liver cancer Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Human Kidney Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Mouse Testis Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Mouse Stomach Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Rat Testis Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GPX4, Human.
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Western Blot; Sample: Human Liver lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: GPX4 knockout 293T cell lysate;
Predicted MW: 22kd
Observed MW: 20kd
Primary Ab: 1μg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: 293T cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Western Blot; Samples: Lane1: Rat Testis lysate; Lane2: Mouse Testis lysate;<br/>Primary Ab: 0.2μg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對GPX4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GPX4。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC994Hu01 | 谷胱甘肽過氧化酶4(GPX4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC994Hu01 | 谷胱甘肽過氧化酶4(GPX4)多克隆抗體 | WB; IHC |
| MAC994Hu21 | 谷胱甘肽過氧化酶4(GPX4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC994Hu | 谷胱甘肽過氧化酶4(GPX4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC994Hu | 谷胱甘肽過氧化酶4(GPX4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC994Hu01 | 谷胱甘肽過氧化酶4(GPX4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biomedicine & Pharmacotherapy | Artesunate alleviates liver fibrosis by regulating ferroptosis signaling pathway [Pubmed: 30551460] |
| Acta Biomater | An all-in-one biomimetic iron-small interfering RNA nanoplatform induces ferroptosis for cancer therapy [Pubmed:35709941] |
| Antioxidants | Vulnerability of Triple-Negative Breast Cancer to Saponin Formosanin C-Induced Ferroptosis [Pubmed:35204181] |
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