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70kDa熱休克蛋白1A(HSPA1A)多克隆抗體

Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

編號(hào)PAB081Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 RPB081Hu01-70kDa熱休克蛋白1A(HSPA1A)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Equus caballus; Equine (Horse，馬), Chicken (Gallus，雞)
應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
下載英文說(shuō)明書中文說(shuō)明書
規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. DAB staining on IHC-P;
Samples: Human Esophagus Cancer Tissue.
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure: FITC staining on IHC-P;
Sample: A549 cells.

Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Heart lysate; Lane4: Mouse Liver lysate; Lane5: Mouse Cerebrum lysate
Primary Ab: 1μg/mL Rabbit Anti-Human HSPA1A Ab
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human HSPA1A Polyclonal Antibody (Catalog PAB081Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)HSPA1A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HSPA1A。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB081Hu02	70kDa熱休克蛋白1A(HSPA1A)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB081Hu01	70kDa熱休克蛋白1A(HSPA1A)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
CAB081Hu01	抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	Loading Control of WB.
PAB081Hu01	70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	WB; IHC; ICC; IP; FCM
PAB081Hu02	70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	WB,IHC
FAB081Hu03	抗70kDa熱休克蛋白1A(HSPA1A)多克隆抗體	FCM
MAB081Hu21	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB; IHC; ICC/IF
MAB081Hu25	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
CAB081Hu22	抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	Loading Control of WB.
MAB081Hu22	70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	WB
FAB081Hu02	抗70kDa熱休克蛋白1A(HSPA1A)單克隆抗體	Flow cytometry.
HEB081Hu	70kDa熱休克蛋白1A(HSPA1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
SEB081Hu	70kDa熱休克蛋白1A(HSPA1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB081Hu	70kDa熱休克蛋白1A(HSPA1A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSB081Hu01	70kDa熱休克蛋白1A(HSPA1A)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".
CGB081Hu01	70kDa熱休克蛋白1A(HSPA1A)cDNA	PCR、蛋白表達(dá)等

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Journal of Neuroinflammation	Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312]
Mediators Inflamm.?	Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980]
Eur J Pharmacol.	Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522]
Pharmacol Rep.	Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743]
Advances in Medical Sciences	A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267]
Archives of Medical Research	Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533]
metabolic brain disease	Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517]
EXPERIMENTAL CELL RESEARCH	Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493]

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