60kD熱休克蛋白(Hsp60)多克隆抗體
Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
- 編號PAA822Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA822Hu01-60kD熱休克蛋白(Hsp60)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;<br/>Samples: Human Kidney Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Human Stomach Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Human Spleen Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;<br/>Sample: Human Spinal cord Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;<br/>Samples: Human MCF7 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human HSPD1 Antibody<br/>Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
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FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20μg/ml Rabbit Anti-Human Hsp60 AntibodySecond Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)
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AF488 staining on IF;<br/>Sample: Hela cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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AF488 staining on IF;<br/>Sample: HepG2 cell<br/>TRITC Phalloidin at 100 nM (shown in pseudo color red)<br/>Primary Ab: 20μg/ml Rabbit Anti-Human Hsp60 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant HSPD1, Human.
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Figure. Western Blot; Sample: Lane1: Rat Liver Tissue; Lane2: Rat Pancreas Tissue; Lane3: Human A549 Cells; Lane4: Human MCF-7 Cells.
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Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: HSPD1 knockout A549 cell lysate;
Predicted MW: 17,61kd
Observed MW: 58kd
Primary Ab: 1μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Porcine Liver lysate; Lane3: Bovine Liver lysate; Lane4: Gallus Liver lysate; Lane5: Canine Liver lysate<br/>Primary Ab: 0.05μg/ml Rabbit Anti-Human Hsp60 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對Hsp60的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別Hsp60。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA822Hu02 | 60kD熱休克蛋白(Hsp60)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA822Hu01 | 60kD熱休克蛋白(Hsp60)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA822Hu01 | 60kD熱休克蛋白(Hsp60)多克隆抗體 | WB; IHC; ICC/IF |
| PAA822Hu02 | 60kD熱休克蛋白(Hsp60)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| FAA822Hu01 | 抗60kD熱休克蛋白(Hsp60)多克隆抗體 | Flow cytometry. |
| LAA822Hu81 | 60kD熱休克蛋白(Hsp60)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA822Hu71 | 60kD熱休克蛋白(Hsp60)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| FAA822Hu41 | 抗60kD熱休克蛋白(Hsp60)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| MAA822Hu22 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu21 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu23 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu24 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu25 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu27 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA822Hu26 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA822Hu02 | 抗60kD熱休克蛋白(Hsp60)單克隆抗體 | Flow cytometry. |
| SEA822Hu | 60kD熱休克蛋白(Hsp60)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA822Hu | 60kD熱休克蛋白(Hsp60)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA822Hu | 60kD熱休克蛋白(Hsp60)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA822Hu01 | 60kD熱休克蛋白(Hsp60)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| Sci Rep | Hsp60 response in experimental and human temporal lobe epilepsy [PubMed: 25801186] |
| Cellular?and Molecular Gastroenterology and Hepatology | Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro [Pubmed: 30878663] |
| Breast Cancer Res Treat | Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer [Pubmed: 32601970] |
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