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肝素酶(HPSE)多克隆抗體

Polyclonal Antibody to Heparanase (HPSE)

HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;

酶與激酶
基因敲除驗(yàn)證

編號PAA711Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA711Hu02-肝素酶(HPSE)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human HPSE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
IHC圖
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant HPA, Human.
Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: HepG2 cell lysate; Lane3: Jurkat cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: HPA knockout HepG2 cell lysate;
Predicted MW: 43,53,55,61kd
Observed MW: 68kd
Primary Ab: 1μg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對HPSE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別HPSE。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA711Hu04	肝素酶(HPSE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu02	肝素酶(HPSE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA711Hu04	肝素酶(HPSE)活性蛋白	Cell?culture;?Activity?Assays.
APA711Hu01	肝素酶(HPSE)活性蛋白	Cell?culture;?Activity?Assays.
EPA711Hu61	肝素酶(HPSE)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA711Hu61	肝素酶(HPSE)活性蛋白	Cell?culture;?Activity?Assays.
RPA711Hu01	肝素酶(HPSE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu03	肝素酶(HPSE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
CPA711Hu21	肝素酶(HPSE)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
PAA711Hu04	肝素酶(HPSE)多克隆抗體	WB; IHC; ICC; IP.
PAA711Hu02	肝素酶(HPSE)多克隆抗體	WB,IHC,ICC/IF
PAA711Hu01	肝素酶(HPSE)多克隆抗體	WB; IHC; ICC; IP.
PAA711Hu03	肝素酶(HPSE)多克隆抗體	WB; IHC; ICC; IP.
FAA711Hu01	抗肝素酶(HPSE)多克隆抗體	Flow cytometry.
LAA711Hu71	肝素酶(HPSE)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAA711Hu23	肝素酶(HPSE)單克隆抗體	WB
MAA711Hu22	肝素酶(HPSE)單克隆抗體	WB; IHC; ICC; IP.
MAA711Hu21	肝素酶(HPSE)單克隆抗體	WB,ICC/IF
SEA711Hu	肝素酶(HPSE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEA711Hu	肝素酶(HPSE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA711Hu	肝素酶(HPSE)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA711Hu01	肝素酶(HPSE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Plos one	PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source]
Journal of Medical Science	Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source]
Int J Clin Exp Pathol	Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183]
Tumour Biology	PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733]
Journal of Medical Science	Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84]
Renal Failure	Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624]

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