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硫氧化還原蛋白(Trx)多克隆抗體

Polyclonal Antibody to Thioredoxin (Trx)

TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein

  • 硫氧化還原蛋白(Trx)多克隆抗體 產(chǎn)品包裝(模擬)
  • 硫氧化還原蛋白(Trx)多克隆抗體 產(chǎn)品包裝(模擬)
  • 硫氧化還原蛋白(Trx)多克隆抗體 FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human Trx Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • PAA702Hu01.jpg Figure. Western Blot; Sample: Recombinant Trx, Human.
  • 硫氧化還原蛋白(Trx)多克隆抗體 Figure. Western Blot; Sample: Lane1: Human hepG2 Cells; Lane2: Human MCF7 Cells; Lane3: Human Hela Cells.
  • 硫氧化還原蛋白(Trx)多克隆抗體 HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human Trx Polyclonal Antibody (Catalog # PAA702Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • 硫氧化還原蛋白(Trx)多克隆抗體 Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human Trx Polyclonal Antibody (Catalog PAA702Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)Trx的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別Trx。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

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編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA702Hu01 硫氧化還原蛋白(Trx)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA702Hu01 硫氧化還原蛋白(Trx)多克隆抗體 WB; IHC; ICC; IP; FCM
FAA702Hu01 抗硫氧化還原蛋白(Trx)多克隆抗體 Flow cytometry.
LAA702Hu81 硫氧化還原蛋白(Trx)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA702Hu71 硫氧化還原蛋白(Trx)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA702Hu25 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu27 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu26 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu29 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu28 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu22 硫氧化還原蛋白(Trx)單克隆抗體 WB
MAA702Hu21 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu23 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
MAA702Hu24 硫氧化還原蛋白(Trx)單克隆抗體 WB; IHC; ICC; IP.
FAA702Hu08 抗硫氧化還原蛋白(Trx)單克隆抗體 Flow cytometry.
FAA702Hu06 抗硫氧化還原蛋白(Trx)單克隆抗體 Flow cytometry.
FAA702Hu04 抗硫氧化還原蛋白(Trx)單克隆抗體 Flow cytometry.
FAA702Hu02 抗硫氧化還原蛋白(Trx)單克隆抗體 Flow cytometry.
SEA702Hu 硫氧化還原蛋白(Trx)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA702Hu 硫氧化還原蛋白(Trx)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA702Hu 硫氧化還原蛋白(Trx)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Human Reproduction The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis [OxfordJournals: source]
Exp Ther Med. Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus [Pubmed:Pmc4061188]
Reproduction Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627]
Free Radical Research Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress [Pubmed:25564095]
Antioxidants Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. [Pubmed:26797643]
FASEB?Journal Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the?… [Pubmed: 30351992]
World?Journal?of?Gastrointestinal?Oncology Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer [Doi: 10.4251/wjgo.v11.i2.117]
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479]
European Journal of Pharmacology Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitus [Pubmed: 31351037]
Journal of Clinical Medicine Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
HISTOCHEMISTRY AND CELL BIOLOGY Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response [Pubmed: 33161477]
Journal of Cosmetic Dermatology Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583]
Heliyon Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats [34159266]
Hum Exp Toxicol Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat [34155936]
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