Smad同源物3(SMAD3)多克隆抗體
Polyclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- 編號PAC123Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPC123Hu01-Smad同源物3(SMAD3)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Figure. Western Blot; Sample: Recombinant Smad3, Human.
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Western Blot; Sample: Rat Pancreas lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: Smad3 knockout PC3 cell lysate;
Predicted MW: 26,36,43,48kd
Observed MW: 43kd
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human PC3 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Smad3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對SMAD3的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別SMAD3。
用法
Western blottin:g: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC123Hu01 | Smad同源物3(SMAD3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Smad同源物3(SMAD3)多克隆抗體 | WB; IHC; ICC; IP. |
| FAC123Hu01 | 抗Smad同源物3(SMAD3)多克隆抗體 | Flow cytometry. |
| MAC123Hu21 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC123Hu22 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC123Hu23 | Smad同源物3(SMAD3)單克隆抗體 | WB; IHC; ICC; IP. |
參考文獻
| 雜志 | 參考文獻 |
| D￡?ˉ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model [] |
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