凝溶膠蛋白(GSN)多克隆抗體
Polyclonal Antibody to Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- 編號(hào)PAA372Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA372Mu01-凝溶膠蛋白(GSN)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 583 ¥ 1361 ¥ 1944 ¥ 4860 ¥ 19440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Uterus Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse GS Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Mouse Spleen Tissue; <br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse GS Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Mouse Pancreas Tissue; <br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse GS Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse GSN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant Gelsolin, Mouse.
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Western Blot; Samples: Lane1: Mouse Lung lysate; Lane2: Human Serum;
Primary Ab: 3μg/ml Rabbit Anti-Mouse GSN Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: U2OS cell lysate; Lane2: Mouse Serum;
Primary Ab: 0.3μg/ml Rabbit Anti-Mouse GSN Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)GSN的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別GSN。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA372Mu01 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA372Mu02 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA372Mu02 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA372Mu01 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA372Mu | 凝溶膠蛋白(GSN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA372Mu | 凝溶膠蛋白(GSN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy [SpringerLink: 3585r8h46q7r0171] |
| Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury [BioMed: 17422094] |
| Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia [Pubmed: 24239294] |
| The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases [Pubmed: 24565402] |
| BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study [Pubmed:Pmc4132929] |
| Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury [Pubmed:25164111] |
| Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease [PubMed: 25864780] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. [pubmed:28365030] |
| Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720] |
| World?Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children [Pubmed:29427164] |
| Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various?… [Pubmed:35327525] |
