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O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體

Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 產(chǎn)品包裝(模擬)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 產(chǎn)品包裝(模擬)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 DAB staining on IHC-P;
    Sample: Porcine Testis Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC027Hu01.jpg Figure. Western Blot; Sample: Recombinant MGMT, Human.
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 Western Blot; Sample: Jurkat cell lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Human MGMT Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 Knockout Varification: <br/>Lane 1: Wild-type Jurkat cell lysate; <br/>Lane 2: MGMT knockout Jurkat cell lysate; <br/>Predicted MW: 21kd <br/>Observed MW: 22kd<br/>Primary Ab: 3μg/ml Rabbit Anti-Human MGMT Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)MGMT的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別MGMT。

用法

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPC027Hu01 O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC027Hu01 O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 WB,IHC
MAC027Hu22 O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)單克隆抗體 WB; IHC; ICC; IP.
SEC027Hu O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC027Hu O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Biotechnology and Bioengineering Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity [PubMed: 23125026]
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