磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)多克隆抗體
Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
PI3K; PI3Kbeta; PIK3C1; p110-BETA; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
- 編號(hào)PAJ829Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPJ829Hu02-磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue Primary Ab: 30μg/ml Rabbit Anti-Human PIK3Cb Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 Cells;
Primary Ab: 10μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human PIK3Cb Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant PIK3Cb, Human.
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: HepG2 cell lysate<br/>Primary Ab: 1μg/ml Rabbit Anti-Human PIK3Cb Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: HepG2 cell lysate;<br/>Primary Ab: 0.1μg/ml Rabbit Anti-Human PIK3Cb Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Human THP1 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human PIK3Cb Polyclonal Antibody (Catalog PAA624Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)PIK3Cb的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別PIK3Cb。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunofluorescence:5-30μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ829Hu01 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ829Hu02 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ829Hu03 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ829Hu02 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)多克隆抗體 | WB; IHC; IF; FCM |
| PAJ829Hu03 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)多克隆抗體 | WB; IHC; ICC; IP. |
| PAJ829Hu01 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)多克隆抗體 | WB; IHC; ICC/IF;FCM |
| LAJ829Hu71 | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEJ829Hu | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ829Hu | 磷酸肌醇-3-激酶催化亞基β肽(PIK3Cb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Genetic Engineering and Biotechnology | Encapsulated VEGF 121-PLA microparticles promote angiogenesis in human endometrium stromal cells [33523322] |
