S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體
Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)
MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
- 編號PAB792Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB792Mu01-S100鈣結(jié)合蛋白A8(S100A8)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.85mg/ml
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse S100A8 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Spleen Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Lung Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Colon Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse S100A8 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant S100A8, Mouse.
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Western Blot; Sample: Lane1: Mouse Spleen lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Skin lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Skin lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Saliva
Primary Ab: 0.1μg/ml Rabbit Anti-Mouse S100A8 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對S100A8的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別S100A8。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB792Mu81 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB792Mu71 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB792Mu21 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB792Mu | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB792Mu | S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X] |
| American Journal of Reproductive Immunology | Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702] |
| J Immunol | S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915] |
| PLoS?One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680] |
| Journal?of?Alzheimer's?Disease | Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324] |
| ?Journal of Functional Foods | Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208] |
| International?Journal?of?Molecular?Medicine | Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993] |
| European?review for?medical?and pharmacological?sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169] |
| Foodborne Pathogens and Disease | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394] |
| Kidney International Reports | Systemic Inflammation Precedes Microalbuminuria in Diabetes [] |
| Cancer Management and Research | iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis [] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in [] |
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