分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體
Monoclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)
FRP-4; FRPHE; Frizzled protein, human endometrium
- 編號(hào)MAF878Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))5#
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPF878Hu01-分泌型卷曲相關(guān)蛋白4(SFRP4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 666 ¥ 1554 ¥ 2220 ¥ 5550 ¥ 22200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P;<br/>Sample: Human Colon Tissue; <br/>Primary Ab: 10μg/ml Mouse Anti-Human SFRP4 Antibody<br/>Second Ab: 2μg/ml?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)<br/>Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2μg/ml?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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DAB staining on IHC-P;<br/>Sample: Rat Colon Tissue<br/>Primary Ab: 30μg/ml Mouse Anti-Human SFRP4 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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DAB staining on IHC-P;<br/>Sample: Rat Uterus Tissue<br/>Primary Ab: 30μg/ml Mouse Anti-Human SFRP4 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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AF488 staining on IF;<br/>Sample: A549 cell<br/>Primary Ab: 30μg/ml Mouse Anti-Human SFRP4 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu11)
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Figure. Western Blot; Sample: Recombinant SFRP4, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Heart lysate; Lane4: SKOV3 cell lysate
Primary Ab: 2μg/ml Mouse Anti-Human SFRP4 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)SFRP4的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別SFRP4。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPF878Hu03 | 分泌型卷曲相關(guān)蛋白4(SFRP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF878Hu02 | 分泌型卷曲相關(guān)蛋白4(SFRP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF878Hu01 | 分泌型卷曲相關(guān)蛋白4(SFRP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF878Hu03 | 分泌型卷曲相關(guān)蛋白4(SFRP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF878Hu02 | 分泌型卷曲相關(guān)蛋白4(SFRP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF878Hu01 | 分泌型卷曲相關(guān)蛋白4(SFRP4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAF878Hu71 | 分泌型卷曲相關(guān)蛋白4(SFRP4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAF878Hu22 | 分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體 | WB; IHC; ICC/IF |
| MAF878Hu21 | 分泌型卷曲相關(guān)蛋白4(SFRP4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEF878Hu | 分泌型卷曲相關(guān)蛋白4(SFRP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF878Hu | 分泌型卷曲相關(guān)蛋白4(SFRP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Endocrine Journal | Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance [PubMed: 26041556] |
| Horm Metab Res. | Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes Mellitus [Pubmed:26882051] |
| Obesity?(Silver Spring).? | Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity [pubmed:27768254] |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans [Pubmed:29427376] |
| Novel marker for gestational diabetes [patent:US20180059122A1] | |
| British?Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile [Pubmed: 30375290] |
| American Journal of?Cancer?Research | SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma [Pubmed: 30906634] |
| Diabetologia | Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic?… [Pubmed: 31011777] |
| PLoS One | First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus [Pubmed: 33206702] |
| Gastric Cancer | SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence [33277667] |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop?… [] |
