拓?fù)洚悩?gòu)酶Ⅱ(TOP2)多克隆抗體
Polyclonal Antibody to Topoisomerase II (TOP2)
DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
- 編號PAA792Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA792Hu01-拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Tonsil Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human TOP2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human TOP2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant TOP2, Human.
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Western Blot; Sample: Human Serum;
Primary Ab: 1μg/ml Rabbit Anti-Human TOP2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對TOP2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別TOP2。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA792Hu01 | 拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA792Hu01 | 拓?fù)洚悩?gòu)酶Ⅱ(TOP2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA792Hu22 | 拓?fù)洚悩?gòu)酶Ⅱ(TOP2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA792Hu | 拓?fù)洚悩?gòu)酶Ⅱ(TOP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA792Hu | 拓?fù)洚悩?gòu)酶Ⅱ(TOP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II [pubmed:27852319] |
| Bioorganic & Medicinal Chemistry | Synthesis of pharmacologically important naphthoquinones and anticancer activity of 2-benzyllawsone through DNA topoisomerase-II inhibition. [pubmed:28094224] |
| European Journal of Medicinal Chemistry | Antiproliferative activity of diarylnaphthylpyrrolidine derivative via dual target inhibition [Pubmed: 31884407] |
| Int J Mol Sci | 2-{5-[(Z,2Z)-2-Chloro-3-(4-nitrophenyl)-2-propenylidene]-4-oxo-2-thioxothiazolidin-3-yl}-3-methylbutanoic Acid as a Potential Anti-Breast Cancer Molecule [Pubmed:35456915] |
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