泛素羧基端酯酶L1(UCHL1)多克隆抗體
Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 編號PAG945Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPG945Hu01-泛素羧基端酯酶L1(UCHL1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: A549 cells.
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Figure. Western Blot; Sample: Recombinant UCHL1, Human.
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Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Porcine Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human A549 Cells.
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Human SH-SY5Y cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human UCHL1 Polyclonal Antibody (Catalog PAC030Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對UCHL1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別UCHL1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPG945Hu01 | 泛素羧基端酯酶L1(UCHL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG945Hu01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體 | WB; IHC; ICC; IP. |
| FAG945Hu01 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體 | Flow cytometry. |
| FAG945Hu41 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| FAG945Hu51 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(別藻藍蛋白標(biāo)記) | Flow cytometry. |
| FAG945Hu81 | 抗泛素羧基端酯酶L1(UCHL1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| MAG945Hu21 | 泛素羧基端酯酶L1(UCHL1)單克隆抗體 | WB; IHC; ICC; IP. |
| FAG945Hu02 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體 | Flow cytometry. |
| FAG945Hu42 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| FAG945Hu52 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(別藻藍蛋白標(biāo)記) | Flow cytometry. |
| FAG945Hu82 | 抗泛素羧基端酯酶L1(UCHL1)單克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| SEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEG945Hu | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEG945Hu | 抗泛素羧基端酯酶L1抗體(Anti-UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMG945Hu | 泛素羧基端酯酶L1(UCHL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSG945Hu01 | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
| Intensive?Care?Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |
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