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載脂蛋白A1(APOA1)重組蛋白

Recombinant Apolipoprotein A1 (APOA1)

Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

編號RPA519Hu01
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源原核表達
宿主E.coli
內(nèi)毒素水平<1.0EU per 1μg (determined by the LAL method).
亞細胞定位分泌
預(yù)測分子量18.5kDa
實際分子量17kDa(差異分析請參閱說明書)
片段與標簽Gln122~Gln267 with N-terminal His Tag
緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
性狀凍干粉
純度> 90%
等電點6.4
應(yīng)用 Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。
下載英文說明書中文說明書
規(guī)格 10μg50μg 200μg 1mg 5mg
價格 ¥ 720 1800 ¥ 1800 ¥ 3600 ¥ 10800 ¥ 27000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Figure . Gene Sequencing (extract)

Figure. SDS-PAGE

通過ISO 9001、ISO 13485質(zhì)量體系認證

序列

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

聚丙烯酰胺凝膠電泳(PAGE)實驗服務(wù)

增值服務(wù)

去內(nèi)毒素試劑盒
 BCA蛋白定量檢測試劑盒
 蛋白標記定制服務(wù)
蛋白分子量MARKER
重組蛋白定制服務(wù)
單克隆抗體定制服務(wù)
多克隆抗體定制服務(wù)
蛋白活性測定實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
緩沖液
 內(nèi)毒素去除試劑盒Ⅱ
實時熒光定量PCR實驗服務(wù)
棘突蛋白受體結(jié)合結(jié)構(gòu)域
 蛋白G
蛋白 A

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA519Hu01	載脂蛋白A1(APOA1)活性蛋白	Cell?culture;?Activity?Assays.
APA519Hu61	載脂蛋白A1(APOA1)活性蛋白	Cell?culture;?Activity?Assays.
RPA519Hu01	載脂蛋白A1(APOA1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPA519Hu61	載脂蛋白A1(APOA1)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA519Hu01	載脂蛋白A1(APOA1)多克隆抗體	WB; IHC; ICC; IP.
LAA519Hu71	載脂蛋白A1(APOA1)多克隆抗體(生物素標記)	WB; IHC; ICC.
MAA519Hu21	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC
MAA519Hu24	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
MAA519Hu26	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
MAA519Hu23	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
MAA519Hu25	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
MAA519Hu29	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
RAA519Hu21	載脂蛋白A1(APOA1)重組抗體	WB; IHC; ICC.
RAA519Hu27	載脂蛋白A1(APOA1)重組抗體	WB; IF; ICC; IHC; IP; FCM.
MAA519Hu22	載脂蛋白A1(APOA1)單克隆抗體	WB; IHC; ICC; IP.
FAA519Hu02	抗載脂蛋白A1(APOA1)單克隆抗體	Flow cytometry.
LAA519Hu76	載脂蛋白A1(APOA1)單克隆抗體(生物素標記)	WB; IHC; ICC.
SEA519Hu	載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEA519Hu	抗載脂蛋白A1抗體(Anti-APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antibody Detection.
SCA519Hu	載脂蛋白A1(APOA1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMA519Hu	載脂蛋白A1(APOA1)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
PSA519Hu01	載脂蛋白A1(APOA1)抗體對	ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA519Hu01	載脂蛋白A1(APOA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志	參考文獻
European Journal of Applied Physiology	Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515]
Official Journal of the INWR	Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source]
Biochemical and Biophysical Research Communications	Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X]
World Journal of Sport Sciences	The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source]
Biochimica et Biophysica Acta	Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Biomarkers	The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349]
Journal of the American Heart Association	MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673]
PLoS ONE	Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source]
Molecular Genetics and Metabolism	DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041]
Endocrinology	Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302]
Molecular Genetics and Metabolism Reports	Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026]
J Psychopharmacol	Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619]
Biochim Biophys Acta	Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Pharmacol Res Perspect	Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234]
Clin Exp Nephrol	Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
Oncogene	Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300]
Oncogene	Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300]
Allergy	Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321]
Journal of Chromatography A	Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
11	High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837]
Biomedical Science Letters	Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice [10.15616/BSL.2017.23.2.80]
ACS?Biomaterials Science?&?Engineering	Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021]
Food?Research International	Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541]
Journal of The Science of Food and Agriculture	Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773]
Toxicon	A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869]
European?Journal of Pharmacology	The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981]
European Journal of Preventive Cardiology	High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266]
Diagnostics	Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
VETERINARY PATHOLOGY	Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Nat Commun	GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Cardiovasc Toxicol	Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258]
Peptides	Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638]
Journal of Functional Foods	Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages?… []

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