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成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)

CLIA Kit for Fibroblast Growth Factor 21 (FGF21)

  • 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) 實(shí)驗(yàn)結(jié)果圖
  • CCC918Ra.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)成纖維細(xì)胞生長(zhǎng)因子21(FGF21),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的成纖維細(xì)胞生長(zhǎng)因子21(FGF21)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 95-104 101
heparin plasma(n=5) 91-98 94

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的成纖維細(xì)胞生長(zhǎng)因子21(FGF21),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中成纖維細(xì)胞生長(zhǎng)因子21(FGF21)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 79-94% 78-95% 80-88%
EDTA plasma(n=5) 89-101% 80-102% 78-104% 79-99%
heparin plasma(n=5) 97-105% 98-105% 79-89% 83-105%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)50µL,
    加入50µL檢測(cè)液A(臨用前配制);
    37°C溫育1小時(shí)。
3. 洗板3次;
4. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分鐘;
7. 讀數(shù)。

實(shí)驗(yàn)原理

本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本),待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物,然后加入HRP標(biāo)記的親和素,再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的成纖維細(xì)胞生長(zhǎng)因子21(FGF21)呈負(fù)相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位(RLU),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APC918Ra01 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)活性蛋白 Cell?culture;?Activity?Assays.
RPC918Ra02 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPC918Ra61 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPC918Ra01 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC918Ra02 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)多克隆抗體 WB; IHC; ICC; IP.
PAC918Ra01 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)多克隆抗體 WB; IHC; ICC; IP.
MAC918Ra21 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)單克隆抗體 WB; IHC; ICC; IP.
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CEC918Ra 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEC918Ra 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) Enzyme-linked immunosorbent assay for Antigen Detection.
CCC918Ra 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC918Ra 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PGC918Ra01 成纖維細(xì)胞生長(zhǎng)因子21(FGF21)引物對(duì) PCR

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Lipids in Health and Disease Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs [Lipidworld: source]
Endocrinology Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression [Endocrine:Source]
Biochemical and Biophysical Research Communications Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH [Pubmed:25449271]
Free Radic Biol Med Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway [PubMed: 25843656]
Journal of diabetes and its complications Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484]
American journal of the medical sciences Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839]
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555]
Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим?… []
Physiol Res Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. [Pubmed: 32469231]
Chinese Journal of Osteoporosis 2 型糖尿病患者血清FGF21, 骨連結(jié)蛋白和骨轉(zhuǎn)換指標(biāo)的相關(guān)性研究. []
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
Nutrients The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose []
Medicine (Baltimore) Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort [34918690]
Annals of Agricultural and Environmental Medicine Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis [34969228]
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074]
Lab Invest Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury [Pubmed:35488034]
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377]
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients []
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