国产成人三级网址在线看_日韩高清在线毛片_精品国内自产拍在线观看尤物_欧美精品 亚洲日韩_成人黄色影片在线看_国产一区二区播放_国产午夜理论最新电影_日本伊人久久综合_亚洲国产网站视频在线播放_日韩人妻系列无码专区

巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(化學(xué)發(fā)光免疫分析法)

CLIA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

  • 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) 實驗結(jié)果圖
  • SCA092Hu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測巨噬細(xì)胞炎性蛋白1α(MIP1a),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的巨噬細(xì)胞炎性蛋白1α(MIP1a)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 94-101 98
heparin plasma(n=5) 84-99 90

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的巨噬細(xì)胞炎性蛋白1α(MIP1a),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中巨噬細(xì)胞炎性蛋白1α(MIP1a)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 81-90% 91-99% 85-94% 78-98%
EDTA plasma(n=5) 83-97% 86-95% 89-99% 95-103%
heparin plasma(n=5) 90-98% 85-102% 80-104% 86-97%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100μL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數(shù)。

實驗原理

將巨噬細(xì)胞炎性蛋白1α(MIP1a)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的巨噬細(xì)胞炎性蛋白1α(MIP1a)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的巨噬細(xì)胞炎性蛋白1α(MIP1a)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的巨噬細(xì)胞炎性蛋白1α(MIP1a)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位(RLU),計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA092Hu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA092Hu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 Cell?culture;?Activity?Assays.
APA092Hu61 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 Cell?culture;?Activity?Assays.
EPA092Hu61 巨噬細(xì)胞炎性蛋白1α(MIP1a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA092Hu51 巨噬細(xì)胞炎性蛋白1α(MIP1a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA092Hu07 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 WB; IHC; ICC; IP.
PAA092Hu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 WB; IHC; ICC; IP.
PAA092Hu06 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 WB; IHC; ICC; IP.
MAA092Hu22 巨噬細(xì)胞炎性蛋白1α(MIP1a)單克隆抗體 WB; IHC; ICC; IP.
SEA092Hu 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA092Hu 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA092Hu 巨噬細(xì)胞炎性蛋白1α(MIP1a)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA092Hu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".
PGA092Hu01 巨噬細(xì)胞炎性蛋白1α(MIP1a)引物對 PCR

參考文獻

雜志 參考文獻
Inhalation Toxicology Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans [Pubmed: 24044680]
Scientific Reports The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204]
ActaMedicaMediterranea CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH … [57e97ac908aeb34bc08fd41a.pdf]
National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396]
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder [10.1016/j.pharep.2017.08.008]
NPG?Asia?Materials Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved?… [Articles:am2017225]
Journal of Immunology?Research Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat [Pubmed:29682587]
Journal of Immunology?Research Cathelicidin LL-37 affects surface and intracellular Toll-like receptor expression in tissue mast cells [Pubmed:29670923]
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without?… [Pubmed:29339257]
Journal?of?Food?Science CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 Pathway [Doi: 10.1111/1750-3841.14346]
Biochemical and Biophysical Research Communications The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792]
LIFE SCIENCES Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274]
ACTA PHARMACOLOGICA SINICA α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in mice [Pubmed: 32973325]
Life Sci PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102]
Nature Communications A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752]
Neoplasia PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342]
留言咨詢