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色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法)

CLIA Kit for Pigment Epithelium Derived Factor (PEDF)

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

  • 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) 實驗結果圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測色素上皮衍生因子(PEDF)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的色素上皮衍生因子(PEDF)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 83-105 89
EDTA plasma(n=5) 79-90 83
heparin plasma(n=5) 92-99 96

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的色素上皮衍生因子(PEDF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中色素上皮衍生因子(PEDF)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-105% 78-97% 80-103% 93-102%
EDTA plasma(n=5) 88-102% 78-101% 90-98% 90-103%
heparin plasma(n=5) 78-91% 81-92% 81-90% 97-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。

實驗原理

將色素上皮衍生因子(PEDF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的色素上皮衍生因子(PEDF)與連接于固相載體上的抗體結合，然后加入生物素化的色素上皮衍生因子(PEDF)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的色素上皮衍生因子(PEDF)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。

相關產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
RPB972Hu01 色素上皮衍生因子(PEDF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB972Hu01 色素上皮衍生因子(PEDF)多克隆抗體 WB; IHC; ICC; IP.
MAB972Hu22 色素上皮衍生因子(PEDF)單克隆抗體 WB; IHC; ICC; IP.
SEB972Hu 色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB972Hu 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB972Hu 色素上皮衍生因子(PEDF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB972Hu01 色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Molecular Therapy A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144]
American Journal of Nephrology Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326]
34 Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source]
Heart and Education in Heart The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source]
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442]
Cardiology. The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856]
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source]
Clinical Science Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221]
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377]
Investigative ophthalmoloey & visual science The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822]
Scientific reports VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556]
國際眼科雜志 色素上皮衍生因子修飾的人臍帶間充質干細胞構建方法 [201707007]
Investigative?Ophthalmology?&?Visual?Science PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136]
International Eye Science 高海拔地區(qū)新生血管性青光眼不同治療方式的療效及對房水中PEDF, VEGF 水平的影響 [2018/2/201802020.pdf]
Domestic?Animal?Endocrinology Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402]
Medical?Science?Monitor Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a?… [Pubmed:29574467]
Chemosphere Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100]
Biological?Trace?Element?Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020]
International?Urology?and Nephrology Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192]
International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807]
Journal of the American Heart Association Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388]
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962]
Hum Cell Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ?- [33768511]
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