酪氨酸羥化酶(TH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Tyrosine Hydroxylase (TH)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
- 編號(hào)SCB438Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)2h, 40min
- 檢測(cè)范圍13.7-10,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于5.6pg/mL.
- 樣本類(lèi)型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 3830 ¥ 5472 ¥ 24624 ¥ 46512 ¥ 383040
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)酪氨酸羥化酶(TH)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的酪氨酸羥化酶(TH)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-98 | 90 |
| EDTA plasma(n=5) | 87-105 | 101 |
| heparin plasma(n=5) | 90-97 | 93 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的酪氨酸羥化酶(TH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中酪氨酸羥化酶(TH)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-104% | 96-104% | 95-103% | 85-98% |
| EDTA plasma(n=5) | 89-96% | 78-88% | 80-94% | 99-105% |
| heparin plasma(n=5) | 98-105% | 88-95% | 82-92% | 92-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將酪氨酸羥化酶(TH)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的酪氨酸羥化酶(TH)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的酪氨酸羥化酶(TH)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的酪氨酸羥化酶(TH)呈正相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位（RLU），計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB438Ra01 | 酪氨酸羥化酶(TH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB438Ra01 | 酪氨酸羥化酶(TH)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB438Ra71 | 酪氨酸羥化酶(TH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB438Ra25 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB438Ra21 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB438Ra22 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB438Ra27 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB438Ra23 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB438Ra24 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB438Ra | 酪氨酸羥化酶(TH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB438Ra | 酪氨酸羥化酶(TH)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB438Ra | 酪氨酸羥化酶(TH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB438Ra01 | 酪氨酸羥化酶(TH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| PLOS ONE | Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| Therapeutics?and?Clinical?Risk?Management | Elevated?blood?plasma?levels?of?epinephrine,?norepinephrine,?tyrosine?hydroxylase, TGFβ1, and TNFα?associated?with?high-altitude?pulmonary?edema?in an Indian population. [pubmed:27540296] |
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