降鈣素基因相關(guān)肽(CGRP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Calcitonin Gene Related Peptide (CGRP)
CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
- 編號(hào)KSA876Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍24.69-2,000pg/mL
- 可適靈敏度
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對(duì)降鈣素基因相關(guān)肽(CGRP)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA876Ra01 | 降鈣素基因相關(guān)肽(CGRP)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA876Ra01 | 降鈣素基因相關(guān)肽(CGRP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA876Ra21 | 降鈣素基因相關(guān)肽(CGRP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA876Ra01 | 降鈣素基因相關(guān)肽(CGRP)多克隆抗體 | WB |
MAA876Ra21 | 降鈣素基因相關(guān)肽(CGRP)單克隆抗體 | WB; IHC; ICC; IP. |
CEA876Ra | 降鈣素基因相關(guān)肽(CGRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA876Ra | 降鈣素基因相關(guān)肽(CGRP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA876Ra | 降鈣素基因相關(guān)肽(CGRP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA876Ra11 | 降鈣素基因相關(guān)肽(CGRP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Evidence-Based Complementary and Alternative Medicine | Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. [Hindawi: 190370] |
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Journal of Clinical Investigation | CNS myeloid cells critically regulate heat hyperalgesia [Pubmed:29634489] |
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BioRxiv?-?the?preprint?server?for?biology | RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis [10.1101:357582] |
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JOURNAL OF PERIODONTOLOGY | Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine [Pubmed: 31070784] |
Molecular and Cellular Biochemistry | Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling [Pubmed: 31420791] |
Journal of Headache and Pain | Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion?… [] |
Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455] |
Europe PMC | Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion?… [Pubmed: 31375062] |
Clinical Chemistry and Laboratory Medicine | Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache [Pubmed: 32286239] |
MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099] |
Scientific Reports | Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs [33441852] |
Nature Communications | Perirenal adipose afferent nerves sustain pathological high blood pressure in rats [Pubmed:35668093] |
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