輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Neurofilament, Light Polypeptide (NEFL)
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
- 編號(hào)KSE038Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.057ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
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特異性
試劑盒中的抗體對(duì)輕肽神經(jīng)絲蛋白(NEFL)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPE038Hu03 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPE038Hu02 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPE038Hu21 | 輕肽神經(jīng)絲蛋白(NEFL)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAE038Hu08 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB; IHC; ICC; IP. |
PAE038Hu02 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB; IHC; ICC; IP. |
PAE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體 | WB; IHC; ICC; IP. |
LAE038Hu71 | 輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAE038Hu25 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; ICC; IP. |
MAE038Hu27 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; ICC; IP. |
MAE038Hu23 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; ICC; IP. |
MAE038Hu22 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; ICC; IP. |
MAE038Hu21 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC |
MAE038Hu24 | 輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | WB; IHC; ICC; IP. |
FAE038Hu08 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | Flow cytometry. |
FAE038Hu02 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | Flow cytometry. |
FAE038Hu06 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | Flow cytometry. |
FAE038Hu04 | 抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體 | Flow cytometry. |
SEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCE038Hu | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LME038Hu | 輕肽神經(jīng)絲蛋白(NEFL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSE038Hu01 | 輕肽神經(jīng)絲蛋白(NEFL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers [Pubmed:27126899] |
Acta Neurologica Scandinavica | Effect of carotid endarterectomy on brain damage markers. [pubmed:27126899] |
journal of neuroimmunology | Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications [Pubmed: 31254929] |
Cerebellum | Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia [33893614] |
Medicine (Baltimore) | Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis [34678870] |
J Clin Med | Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients?… [Pubmed:35566558] |
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