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輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體

Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

神經(jīng)科學(xué)

編號MAE038Hu21
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號)C4
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPE038Hu02-輕肽神經(jīng)絲蛋白(NEFL)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/ml
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Rat Cerebrum Tissue; Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Cerebellum Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human NEFL Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate Primary Ab: 0.001μg/ml Mouse Anti-Human NEFL Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Human SH-SY5Y cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human NEFL Monoclonal Antibody (Catalog MAE038Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對NEFL的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別NEFL。

用法

Western blotting: 0.001-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPE038Hu03	輕肽神經(jīng)絲蛋白(NEFL)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPE038Hu02	輕肽神經(jīng)絲蛋白(NEFL)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPE038Hu01	輕肽神經(jīng)絲蛋白(NEFL)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
CPE038Hu21	輕肽神經(jīng)絲蛋白(NEFL)卵白蛋白偶聯(lián)物	Immunogen; SDS-PAGE; WB.
PAE038Hu08	輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體	WB; IHC; ICC; IP.
PAE038Hu02	輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體	WB; IHC; ICC; IP.
PAE038Hu01	輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體	WB; IHC; ICC; IP.
LAE038Hu71	輕肽神經(jīng)絲蛋白(NEFL)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAE038Hu25	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC; ICC; IP.
MAE038Hu27	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC; ICC; IP.
MAE038Hu23	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC; ICC; IP.
MAE038Hu22	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC; ICC; IP.
MAE038Hu21	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC
MAE038Hu24	輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	WB; IHC; ICC; IP.
FAE038Hu08	抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	Flow cytometry.
FAE038Hu02	抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	Flow cytometry.
FAE038Hu06	抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	Flow cytometry.
FAE038Hu04	抗輕肽神經(jīng)絲蛋白(NEFL)單克隆抗體	Flow cytometry.
SEE038Hu	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEE038Hu	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEE038Hu	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
CEE038Hu	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCE038Hu	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LME038Hu	輕肽神經(jīng)絲蛋白(NEFL)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSE038Hu01	輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Acta Neurologica Scandinavica	Effect of carotid endarterectomy on brain damage markers [Pubmed:27126899]
Acta Neurologica Scandinavica	Effect of carotid endarterectomy on brain damage markers. [pubmed:27126899]
journal of neuroimmunology	Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications [Pubmed: 31254929]
Cerebellum	Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia [33893614]
Medicine (Baltimore)	Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis [34678870]
J Clin Med	Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients?… [Pubmed:35566558]

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