国产成人三级网址在线看_日韩高清在线毛片_精品国内自产拍在线观看尤物_欧美精品 亚洲日韩_成人黄色影片在线看_国产一区二区播放_国产午夜理论最新电影_日本伊人久久综合_亚洲国产网站视频在线播放_日韩人妻系列无码专区

谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Alanine Aminotransferase (ALT)

GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1

  • 谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA207Bo.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)谷丙轉(zhuǎn)氨酶(ALT)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的谷丙轉(zhuǎn)氨酶(ALT)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 82-94 89
EDTA plasma(n=5) 81-103 90
heparin plasma(n=5) 89-96 93

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的谷丙轉(zhuǎn)氨酶(ALT)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中谷丙轉(zhuǎn)氨酶(ALT)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-92% 84-99% 78-101% 91-99%
EDTA plasma(n=5) 78-101% 79-98% 98-105% 80-97%
heparin plasma(n=5) 80-101% 79-89% 78-104% 85-97%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將谷丙轉(zhuǎn)氨酶(ALT)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的谷丙轉(zhuǎn)氨酶(ALT)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的谷丙轉(zhuǎn)氨酶(ALT)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的谷丙轉(zhuǎn)氨酶(ALT)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Bos taurus; Bovine (Cattle，牛) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA207Bo01 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA207Bo01 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 WB; IHC; ICC; IP.
MAA207Bo21 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 WB; IHC; ICC; IP.
SEA207Bo 谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA207Bo 谷丙轉(zhuǎn)氨酶(ALT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Cancer Gene Therapy Enhanced antitumor activity by combining an adenovirus harboring ING4 with cisplatin for hepatocarcinoma cells [Nature: 201067]
Endocrinology. Additive effects of nicotine and high-fat diet on hepatic steatosis in male mice. [PubMed: 23093702]
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms [PubMed: PMC3612362]
Proceedings of the National Academy of Sciences Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model [PubMed: PMC3382536]
African Journal of Pharmacy and Pharmacology Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats [Academicjournals: Source]
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074]
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats [ScienceDirect: S0014299914000235]
Eur J Nutr. Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis [Pubmed:25657013]
Experimental and Molecular Pathology Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitis [Pubmed:25681756]
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061]
FNG & Bilim T?p Dergisi The effects of subacute deltamethrin exposure on rat liver: histochemical, immunohistochemical, and biochemical study [xmlui:11446]
Gastroenterol Res Pract. Advanced Studies in Clinical and Experimental Research in Gastroenterology [Pubmed:26955389]
DARU?Journal of Pharmaceutical Sciences Effects of developmental exposure to silver in ionic and nanoparticle form: A study in rats [pubmed:27716437]
Oncotarget Dihydromyricetin?protects?against?liver?ischemia/reperfusion?induced?apoptosis?via?activation?ofFOXO3a-mediated?autophagy. [pubmed:27793014]
Scientific Reports Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302]
?Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485]
Gastroenterology Research and Practice Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model [pubmed:28912805]
PLoS Pathogens Alcohol-associated intestinal dysbiosis impairs pulmonary host defense against Klebsiella pneumoniae. [pubmed:28604843]
FEBS?Open?Bio A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis [Pubmed:29321963]
Rolle von CMKLR1 und dessen Liganden bei der nicht-alkoholischen Fettlebererkrankung [Regensburg:Source]
Cumhuriyet Medical Journal The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injury [DOI:Source]
BMC?Complementary and Alternative?Medicine? Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910]
Scientific Reports Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle [Pubmed: 30787318]
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533]
Biomed Research International Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099]
Frontiers in Immunology CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism [Pubmed: 32158445]
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313]
European Journal of Biology Effects of Tempol in Lipopolysaccharide-Induced Liver Injury []
Pathogens Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus [33925018]
Gastroenterol Res Pract An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment [34804155]
Int Immunopharmacol Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes [34848154]
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074]
Medcomm Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and?… [Pubmed:35548710]
International Immunopharmacology Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433]
留言咨詢