α1-酸性糖蛋白(a1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)
AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1
- 編號SEA816Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍31.2-2,000ng/mL
- 靈敏度最小可檢測劑量小于等于14.1ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測α1-酸性糖蛋白(a1AGP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的α1-酸性糖蛋白(a1AGP)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-103 | 92 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 78-89 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的α1-酸性糖蛋白(a1AGP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中α1-酸性糖蛋白(a1AGP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-96% | 87-95% | 92-104% | 85-95% |
| EDTA plasma(n=5) | 98-105% | 96-104% | 80-99% | 89-96% |
| heparin plasma(n=5) | 78-91% | 85-103% | 96-104% | 96-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將α1-酸性糖蛋白(a1AGP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的α1-酸性糖蛋白(a1AGP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的α1-酸性糖蛋白(a1AGP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的α1-酸性糖蛋白(a1AGP)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA816Ra02 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA816Ra03 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA816Ra01 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA816Ra02 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA816Ra03 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA816Ra01 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA816Ra71 | α1-酸性糖蛋白(a1AGP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA816Ra23 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA816Ra27 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA816Ra25 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | IHC |
| MAA816Ra24 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA816Ra26 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA816Ra21 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA816Ra22 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA816Ra02 | 抗α1-酸性糖蛋白(a1AGP)單克隆抗體 | Flow cytometry. |
| LAA816Ra76 | α1-酸性糖蛋白(a1AGP)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA816Ra | α1-酸性糖蛋白(a1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA816Ra | α1-酸性糖蛋白(a1AGP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA816Ra01 | α1-酸性糖蛋白(a1AGP)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA816Ra01 | α1-酸性糖蛋白(a1AGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| J Reprod Dev.? | An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro [Pubmed:Pmc4219990] |
| Molecular Reproduction and Development | Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro [Pubmed:25123565] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
| Sci Rep.? | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Scientific Reports | An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle [pubmed:28667317] |
| 33 | Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. [pubmed:28927749] |
| Brazilian Journal of Otorhinolaryngology | Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome [Pubmed: 30213594] |
| BioRxiv | Proteome-wide characterization and biomarker identification of intracranial aneurysm [] |
| The FEBS journal | Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer [Pubmed: 31482685] |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… [] |
| A New Biomarker Tool for Risk Stratification in ?°De Novo?± Acute Heart Failure (OROME) [] |
