凝溶膠蛋白(GSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- 編號SEA372Bo
- 物種Bos taurus; Bovine (Cattle,牛) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍7.8-500ng/mL
- 靈敏度最小可檢測劑量小于等于3.6ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3352 ¥ 4788 ¥ 21546 ¥ 40698 ¥ 335160
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特異性
本試劑盒用于檢測凝溶膠蛋白(GSN),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的凝溶膠蛋白(GSN)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 79-104 | 97 |
EDTA plasma(n=5) | 91-104 | 95 |
heparin plasma(n=5) | 91-105 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的凝溶膠蛋白(GSN),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中凝溶膠蛋白(GSN)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-105% | 99-105% | 98-105% | 83-90% |
EDTA plasma(n=5) | 79-91% | 84-98% | 92-101% | 93-101% |
heparin plasma(n=5) | 80-95% | 87-101% | 78-102% | 99-105% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將凝溶膠蛋白(GSN)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的凝溶膠蛋白(GSN)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的凝溶膠蛋白(GSN)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的凝溶膠蛋白(GSN)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Bos taurus; Bovine (Cattle,牛) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA372Bo01 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA372Bo01 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
MAA372Bo21 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
SEA372Bo | 凝溶膠蛋白(GSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA372Bo | 凝溶膠蛋白(GSN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA372Bo01 | 凝溶膠蛋白(GSN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy [SpringerLink: 3585r8h46q7r0171] |
Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury [BioMed: 17422094] |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563] |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia [Pubmed: 24239294] |
The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases [Pubmed: 24565402] |
BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study [Pubmed:Pmc4132929] |
Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury [Pubmed:25164111] |
Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease [PubMed: 25864780] |
Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. [pubmed:28365030] |
Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720] |
World?Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children [Pubmed:29427164] |
Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various?… [Pubmed:35327525] |