心肌肌鈣蛋白I(cTnI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- 編號SEA478Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍31.2-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于12.1pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2352 ¥ 3360 ¥ 15120 ¥ 28560 ¥ 235200
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測心肌肌鈣蛋白I(cTnI)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的心肌肌鈣蛋白I(cTnI)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 91-99 | 94 |
| heparin plasma(n=5) | 78-102 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的心肌肌鈣蛋白I(cTnI)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中心肌肌鈣蛋白I(cTnI)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-97% | 79-93% | 95-102% | 87-104% |
| EDTA plasma(n=5) | 83-92% | 89-101% | 84-102% | 80-105% |
| heparin plasma(n=5) | 80-90% | 91-101% | 82-97% | 96-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將心肌肌鈣蛋白I(cTnI)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的心肌肌鈣蛋白I(cTnI)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的心肌肌鈣蛋白I(cTnI)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的心肌肌鈣蛋白I(cTnI)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA478Hu02 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA478Hu61 | 心肌肌鈣蛋白I(cTnI)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA478Hu01 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA478Hu01 | 心肌肌鈣蛋白I(cTnI)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA478Hu01 | 心肌肌鈣蛋白I(cTnI)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA478Hu71 | 心肌肌鈣蛋白I(cTnI)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA478Hu28 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA478Hu21 | 心肌肌鈣蛋白I(cTnI)重組抗體 | WB; IHC; ICC. |
| MAA478Hu21 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu27 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu23 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu24 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu25 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu29 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu22 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu26 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA478Hu08 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| FAA478Hu06 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| FAA478Hu04 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| FAA478Hu02 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| LAA478Hu74 | 心肌肌鈣蛋白I(cTnI)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA478Hu | 抗心肌肌鈣蛋白I抗體(Anti-cTnI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA478Hu | 心肌肌鈣蛋白I(cTnI)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA478Hu | 心肌肌鈣蛋白I(cTnI)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA478Hu01 | 心肌肌鈣蛋白I(cTnI)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source] |
| Interact Cardiovasc Thorac Surg. | Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model [Pubmed:25205781] |
| Exp Ther Med | Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury [PubMed: 26136887] |
| Heart International | Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction [pubmed:27924211] |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis [S0301-732X2016000300004] |
| PLOS?Pathogens | Cathepsin B aggravates coxsackievirus B3-induced myocarditis through activating the inflammasome and promoting pyroptosis [Pubmed:29360865] |
| Science?of the?Total?Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991] |
| Molecules | Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy [Pubmed:29547569] |
| Molecules | The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a?… [Pubmed:29494523] |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Biological & Pharmaceutical Bulletin | Protective Effects of Shenfu Injection against Myocardial Ischemia-Reperfusion Injury via Activation of eNOS in Rats [Pubmed:29910216] |
| Oncology?Letters | Hydrogen?rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus?activated kinase 2/signal transducer and activator of?… [Pubmed:29928398] |
| BMC?Pharmacology and Toxicology | Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Frontiers in Pharmacology | Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway [Pubmed: 30890937] |
| canadian journal of physiology and pharmacology | Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? [Pubmed: 30840489] |
| Journal?of?Cellular?Physiology | Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981] |
| BIOSENS?BIOELECTRON | Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA?… [Pubmed: 30954926] |
| Life Sciences | Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia [Pubmed: 31102744] |
| Mikrochim Acta | DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label [Pubmed: 31123904] |
| Biosensors and Bioelectronics | DNA nanotetrahedron-assisted electrochemical aptasensor for cardiac troponin I detection based on the co-catalysis of hybrid nanozyme, natural enzyme and artificial?… [] |
| Biofactors | MiR‐183‐5p protects rat hearts against myocardial ischemia/reperfusion injury through targeting VDAC1 [Pubmed: 31618500] |
| Nature Communications | Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb [Pubmed: 32161256] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| Pharmacol Res | Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat [Pubmed: 32942017] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease [Pubmed: 32605184] |
| Eur J Pharmacol | NLRX1 knockout aggravates lipopolysaccharide (LPS)-induced heart injury and attenuates the anti-LPS cardioprotective effect of CYP2J2/11, 12-EET by enhancing … [Pubmed: 32574674] |
| American Journal of Translational Research | Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … [Pubmed: 32509190] |
| Arabian Journal for Science and Engineering | Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats [] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| Hum Exp Toxicol | Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats [33882726] |
| Curr Protoc | Isolation and Culture of Neonatal Murine Primary Cardiomyocytes [34289259] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344] |
| Biomed Pharmacother | Pea (Pisum sativum) peel extract attenuates DOX-induced oxidative myocardial injury [34649330] |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications [34673993] |
| Biomed Res Int | Nicotinic Acid Riboside Regulates Nrf-2/P62-Related Oxidative Stress and Autophagy to Attenuate Doxorubicin-Induced Cardiomyocyte Injury [Pubmed:35242876] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein [Pubmed:35714415] |
| Nutrients | Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 [Pubmed:35215383] |
留言咨詢
