谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Glutathione Peroxidase 1 (GPX1)
GSHPX1; Cellular glutathione peroxidase
- 編號SEA295Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.22ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測谷胱甘肽過氧化酶1(GPX1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的谷胱甘肽過氧化酶1(GPX1)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-103 | 88 |
| EDTA plasma(n=5) | 78-95 | 89 |
| heparin plasma(n=5) | 91-99 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的谷胱甘肽過氧化酶1(GPX1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中谷胱甘肽過氧化酶1(GPX1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 87-101% | 95-102% | 87-95% |
| EDTA plasma(n=5) | 91-101% | 83-92% | 80-97% | 82-93% |
| heparin plasma(n=5) | 89-103% | 90-97% | 96-103% | 78-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將谷胱甘肽過氧化酶1(GPX1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的谷胱甘肽過氧化酶1(GPX1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的谷胱甘肽過氧化酶1(GPX1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的谷胱甘肽過氧化酶1(GPX1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA295Hu01 | 谷胱甘肽過氧化酶1(GPX1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA295Hu01 | 谷胱甘肽過氧化酶1(GPX1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA295Hu22 | 谷胱甘肽過氧化酶1(GPX1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA295Hu | 谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA295Hu | 谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation [Plos:Source] |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098] |
| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer [Pubmed:25550558] |
| Adv Med Sci | Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, [PubMed: 26674569] |
| Applied Biological Research | Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditions [publication:301246301] |
| Cell Biol Int | Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions [fileID:697741] |
| Toxicology Mechanisms and Methods | Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism [pubmed:27599793] |
| Journal of Environmental Research and Public Health | Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas. [pubmed:28574428] |
| Pharmacological Reports | Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder. [pubmed:28958613] |
| CNS Spectrums | Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study [pubmed:29248027] |
| Environmental toxicology and pharmacology | Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress [pubmed:28654832] |
| REVISTA DE CHIMIE | Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditis [pdf:44%20PREDA%20C%207%2017.pdf] |
| Biological Rhythm Research? | Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate [10.1080:09291016.2017.1361584] |
| Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer [] | |
| Kolon Kanserinde Gpx1 Gen Anlat?m?, Serum Gpx1 ve Selenyum Düzeylerinin ?ncelenmesi [] | |
| Alcohol. | Influence of deficient nutrition on trace element status and antioxidant defense system [Pubmed: 1781925] |
| BMC Infectious Diseases | Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching?… [Pubmed: 31703562] |
| LIFE SCIENCES | Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway [Pubmed: 32798557] |
| AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS [] | |
| The Journal of Physiology | In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells [] |
| World Neurosurg | Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ?- [33689849] |
| J Pers Med | Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis [34945751] |
| J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating?… [Pubmed:35281603] |
