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低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

  • 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA798Hu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)低氧誘導(dǎo)因子1α(HIF1a)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的低氧誘導(dǎo)因子1α(HIF1a)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 82-104 86
heparin plasma(n=5) 85-96 93

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的低氧誘導(dǎo)因子1α(HIF1a)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中低氧誘導(dǎo)因子1α(HIF1a)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 78-96% 93-105% 93-101% 97-105%
EDTA plasma(n=5) 80-101% 96-103% 78-94% 87-104%
heparin plasma(n=5) 97-104% 95-105% 86-98% 91-98%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將低氧誘導(dǎo)因子1α(HIF1a)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的低氧誘導(dǎo)因子1α(HIF1a)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的低氧誘導(dǎo)因子1α(HIF1a)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的低氧誘導(dǎo)因子1α(HIF1a)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA798Hu02 低氧誘導(dǎo)因子1α(HIF1a)活性蛋白 Cell?culture;?Activity?Assays.
RPA798Hu01 低氧誘導(dǎo)因子1α(HIF1a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Hu02 低氧誘導(dǎo)因子1α(HIF1a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA798Hu01 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體 WB; IHC; ICC; IP.
PAA798Hu02 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體 WB; IHC; ICC; IP.
LAA798Hu81 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA798Hu71 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA798Hu21 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu23 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu24 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu25 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu22 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu27 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
MAA798Hu29 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 WB; IHC; ICC; IP.
SEA798Hu 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA798Hu 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA798Hu 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Hu 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA798Hu 低氧誘導(dǎo)因子1α(HIF1a)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA798Hu01 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

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