表面活性物質(zhì)關聯(lián)蛋白B(SP-B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Surfactant Protein B (SP-B)
SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein
- 編號SEB622Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.59ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測表面活性物質(zhì)關聯(lián)蛋白B(SP-B)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的表面活性物質(zhì)關聯(lián)蛋白B(SP-B)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 85-104 | 94 |
| heparin plasma(n=5) | 82-105 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的表面活性物質(zhì)關聯(lián)蛋白B(SP-B)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中表面活性物質(zhì)關聯(lián)蛋白B(SP-B)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-98% | 79-103% | 97-105% | 80-102% |
| EDTA plasma(n=5) | 98-105% | 88-95% | 95-102% | 81-102% |
| heparin plasma(n=5) | 78-94% | 89-96% | 82-104% | 95-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將表面活性物質(zhì)關聯(lián)蛋白B(SP-B)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的表面活性物質(zhì)關聯(lián)蛋白B(SP-B)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的表面活性物質(zhì)關聯(lián)蛋白B(SP-B)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的表面活性物質(zhì)關聯(lián)蛋白B(SP-B)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB622Hu01 | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB622Hu02 | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB622Hu01 | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB622Hu22 | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB622Hu21 | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB622Hu | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB622Hu | 表面活性物質(zhì)關聯(lián)蛋白B(SP-B)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery [PubMed: 21736957] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594] |
| 19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study [PubMed: 20650982] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032] |
| Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models [PubMed: PMC3406925] |
| Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project [Pubmed: 23619193] |
| PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure [Pubmed:25514679] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source] | |
| Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study [PubMed: 26310985] |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142] |
| PLoS One | The?Cerebral?Surfactant?System?and Its?Alteration?in?HydrocephalicConditions. [pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations?of?Ventricular?Enlargement?with?Rheologically?Active?SurfactantProteins?in?Cerebrospinal?Fluid. [pubmed:28101052] |
| Frontiers in?Aging?Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370] |
| Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers [pubmed:28467885] |
| Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production [pubmed:28743125] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126] |
| Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649] |
| Respiratory Physiology & Neurobiology? | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan [Pubmed:29548887] |
| Lung? | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934] |
| American?Journal?of?Physiology?Lung?Cellular?andMolecular?Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells [Pubmed: 30211653] |
| Respiratory?Research | Culture of human alveolar epithelial type II cells by sprouting [Pubmed: 30340591] |
