Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Runt Related Transcription Factor 2 (RUNX2)
AML3; CBFA1; CCD1; OSF2; PEBP2A; Acute myeloid leukemia 3 ; Core-binding factor alpha-1; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 A
- 編號SEB011Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.054ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB011Hu01 | Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB011Hu01 | Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB011Hu22 | Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB011Hu | Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB011Hu | Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| International Journal of Cardiology | Valvular osteoclasts in calcification and aortic valve stenosis severity [PubMed: 23452891] |
| BioMed Research International | Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis [Pubmed:27314037] |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … [doi:10.1002] |
| International Journal of Pharmaceutical Sciences | MicroRNA-320a protects against osteoarthritis cartilage degeneration by regulating the expressions of BMI-1 and RUNX2 in chondrocytes [10.1691:ph.2017.6890] |
| Dental Materials Journal | Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. [pubmed:28228629] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
| Biochemical and biophysical research communications | Cereblon (CRBN) deletion reverses streptozotocin induced diabetic osteoporosis in mice [Pubmed:29353038] |
| Journal of?Infection?and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity [Pubmed:29753615] |
| medical science monitor | Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132?… [Pubmed: 30923307] |
| EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats [Pubmed: 33010420] |
| Molecular Medicine Reports | Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3?E1 cells via an autocrine mechanism [Pubmed: 33000198] |
| Effect of Corticopuncture (CP), Photobiomodulation (PBM) and the Combined Method on the Rate of Tooth Movement and Root Resorption: A Molecular, Histological … [] |
