天蚕土豆,兽性总裁的爱奴

S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

編號(hào)SEB792Hu
物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實(shí)驗(yàn)方法雙抗夾心
反應(yīng)時(shí)長(zhǎng)3h
檢測(cè)范圍15.6-1,000pg/mL
靈敏度最小可檢測(cè)劑量小于等于5.6pg/mL.
樣本類(lèi)型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 48T96T 96T*5 96T*10 96T*100
價(jià)格 ¥ 2352 3360 ¥ 3360 ¥ 15120 ¥ 28560 ¥ 235200
欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!

產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

實(shí)驗(yàn)結(jié)果圖

標(biāo)準(zhǔn)曲線圖

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)S100鈣結(jié)合蛋白A8(S100A8)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的S100鈣結(jié)合蛋白A8(S100A8)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	97-105	101
EDTA plasma(n=5)	82-101	88
heparin plasma(n=5)	85-105	95

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的S100鈣結(jié)合蛋白A8(S100A8)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中S100鈣結(jié)合蛋白A8(S100A8)含量的測(cè)定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	80-90%	80-95%	95-105%	99-105%
EDTA plasma(n=5)	80-94%	87-94%	81-105%	84-91%
heparin plasma(n=5)	89-102%	80-99%	85-92%	83-93%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將S100鈣結(jié)合蛋白A8(S100A8)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的S100鈣結(jié)合蛋白A8(S100A8)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的S100鈣結(jié)合蛋白A8(S100A8)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的S100鈣結(jié)合蛋白A8(S100A8)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

贈(zèng)品

ELISA/CLIA實(shí)驗(yàn)服務(wù)

增值服務(wù)

檢測(cè)試劑盒單組分試劑
 ELISA/CLIA專(zhuān)用裂解液
試劑盒質(zhì)控品
酶聯(lián)免疫試劑盒定制服務(wù)
人類(lèi)疾病動(dòng)物模型定制服務(wù)
血清定制服務(wù)
TGFB1活化劑
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
鏈霉親和素
 蛋白快速染色液
 流式熒光發(fā)光法檢測(cè)試劑盒單組份試劑
 鏈霉親和素瓊脂糖微球

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
APB792Hu01	S100鈣結(jié)合蛋白A8(S100A8)活性蛋白	Cell?culture;?Activity?Assays.
RPB792Hu02	S100鈣結(jié)合蛋白A8(S100A8)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB792Hu01	S100鈣結(jié)合蛋白A8(S100A8)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB792Hu01	S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體	WB; IHC; ICC; IP.
PAB792Hu02	S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體	WB; IHC; ICC; IP; FCM
LAB792Hu81	S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
LAB792Hu71	S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAB792Hu21	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC.
MAB792Hu25	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu22	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu24	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; ICC/IF
MAB792Hu27	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu29	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu26	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu28	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
MAB792Hu23	S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	WB; IHC; ICC; IP.
FAB792Hu02	抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	Flow cytometry.
FAB792Hu04	抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	Flow cytometry.
FAB792Hu06	抗S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體	Flow cytometry.
SEB792Hu	S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB792Hu	S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
PSB792Hu01	S100鈣結(jié)合蛋白A8(S100A8)抗體對(duì)	ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB792Hu01	S100鈣結(jié)合蛋白A8(S100A8)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Biochemical and Biophysical Research Communications	High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X]
American Journal of Reproductive Immunology	Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120]
the faseb journal	Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
J Immunol	S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915]
PLoS?One	Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
Journal?of?Alzheimer's?Disease	Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324]
?Journal of Functional Foods	Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208]
International?Journal?of?Molecular?Medicine	Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464]
International journal of molecular sciences	Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology	Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology	Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry	Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
European?review for?medical?and pharmacological?sciences	S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Foodborne Pathogens and Disease	Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394]
Kidney International Reports	Systemic Inflammation Precedes Microalbuminuria in Diabetes []
Cancer Management and Research	iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093]
scandinavian journal of clinical & laboratory investigation	Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE	Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
MOLECULAR VISION	Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227]
university of copenhagen	Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []

留言咨詢