P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for P-Selectin (SELP)
CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
- 編號SEA569Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于27pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1966 ¥ 2808 ¥ 12636 ¥ 23868 ¥ 196560
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測P選擇素(SELP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的P選擇素(SELP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 85-96 | 92 |
| heparin plasma(n=5) | 90-102 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的P選擇素(SELP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中P選擇素(SELP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 90-104% | 88-103% | 85-101% |
| EDTA plasma(n=5) | 98-105% | 98-105% | 91-99% | 97-104% |
| heparin plasma(n=5) | 95-102% | 92-101% | 78-98% | 86-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將P選擇素(SELP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的P選擇素(SELP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的P選擇素(SELP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的P選擇素(SELP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA569Hu02 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA569Hu61 | P選擇素(SELP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA569Hu61 | P選擇素(SELP)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA569Hu03 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA569Hu01 | P選擇素(SELP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA569Hu01 | P選擇素(SELP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA569Hu02 | P選擇素(SELP)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA569Hu01 | 抗P選擇素(SELP)多克隆抗體 | Flow cytometry. |
| LAA569Hu71 | P選擇素(SELP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA569Hu81 | P選擇素(SELP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA569Hu24 | P選擇素(SELP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA569Hu21 | P選擇素(SELP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA569Hu22 | P選擇素(SELP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA569Hu23 | P選擇素(SELP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA569Hu | P選擇素(SELP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA569Hu | P選擇素(SELP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA569Hu | P選擇素(SELP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journalof Vascular Research | Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340] |
| Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
| Materials Science and Engineering: C | MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption [Pubmed:25063132] |
| Blood Coagul Fibrinolysis.? | Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. [Pubmed:25325345] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275] |
| Nutrition?&?Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
| Available at SSRN 3335365? | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers [] |
| Ecotoxicology?and?Environmental?Safety | Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P [] |
| Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449] |
| JOURNAL OF CONTROLLED RELEASE | Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … [Pubmed: 31982435] |
| OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice [Pubmed: 32377309] |
| blood advances | The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis [34559203] |
| Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] |
| Int J Biol Macromol | The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury [Pubmed:35421414] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
