環(huán)加氧酶2(COX 2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Cyclooxygenase-2 (COX 2)
PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2
- 編號SEA699Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于35pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測環(huán)加氧酶2(COX 2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的環(huán)加氧酶2(COX 2)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-99 | 96 |
| EDTA plasma(n=5) | 78-89 | 81 |
| heparin plasma(n=5) | 85-102 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的環(huán)加氧酶2(COX 2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中環(huán)加氧酶2(COX 2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 82-94% | 81-98% | 91-105% |
| EDTA plasma(n=5) | 91-105% | 99-105% | 85-101% | 79-97% |
| heparin plasma(n=5) | 84-105% | 95-103% | 86-105% | 93-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將環(huán)加氧酶2(COX 2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的環(huán)加氧酶2(COX 2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的環(huán)加氧酶2(COX 2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的環(huán)加氧酶2(COX 2)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA699Mu02 | 環(huán)加氧酶2(COX 2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA699Mu01 | 環(huán)加氧酶2(COX 2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA699Mu01 | 環(huán)加氧酶2(COX 2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA699Mu02 | 環(huán)加氧酶2(COX 2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA699Mu81 | 環(huán)加氧酶2(COX 2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA699Mu71 | 環(huán)加氧酶2(COX 2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA699Mu | 環(huán)加氧酶2(COX 2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA699Mu | 環(huán)加氧酶2(COX 2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Evidence-Based Complementary and Alternative Medicine | Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in Carcinogenesis [PubMed: 22888366] |
| Integrative Cancer Therapies | Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits [Pubmed: 24013642] |
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| Journal of Biomechanics | The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis [pubmed:29137729] |
| Journal of Global Pharma Technology | Inflammatory Response of Annona Muricata Linn Leaves Extract in Colorectal Cancer Patients [NCT02439580] |
| International?Journal?of?Molecular?Sciences | High Expression of IL-1RI and EP2 Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs—Osthole in Inhibition COX-2 [Pubmed: 30620999] |
| International Immunopharmacology | A novel concept of immunological and allergy interactions in Autism Spectrum Disorders: Molecular, anti-inflammatory effect of osthole [Pubmed: 30953868] |
| Reports of Biochemistry & Molecular Biology | Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization [] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
