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催乳素(PRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Prolactin (PRL)

LTH; Luteotropic Hormone

  • 催乳素(PRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 催乳素(PRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 催乳素(PRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA846Cp.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)催乳素(PRL)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的催乳素(PRL)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-103 98
EDTA plasma(n=5) 87-104 94
heparin plasma(n=5) 90-97 93

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的催乳素(PRL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中催乳素(PRL)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 91-101% 96-104% 93-101%
EDTA plasma(n=5) 94-101% 86-98% 78-103% 96-105%
heparin plasma(n=5) 94-103% 82-94% 81-96% 82-99%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將催乳素(PRL)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的催乳素(PRL)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的催乳素(PRL)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的催乳素(PRL)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Capra hircus; Caprine (Goat，山羊) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA846Cp01 催乳素(PRL)活性蛋白 Cell?culture;?Activity?Assays.
RPA846Cp01 催乳素(PRL)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
SEA846Cp 催乳素(PRL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA846Cp 催乳素(PRL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
The Journal of Biological Chemistry In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland [Jcb: 39297]
Journal of Clinical Neuroscience TLR9 expression is associated with prognosis in patients with glioblastoma multiforme [ScienceDirect: S0967586811003572]
Sensors and Actuators B: Chemical Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes [Sciencedirect:S0925400514000720]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Journal?of?Dairy?Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503]
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells [pubmed:29096715]
Stem?Cell?Research?&?Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Toxicology and?Applied?Pharmacology The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance [Pubmed:29551354]
Journal of Cellular Biochemistry The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway [Pubmed:29388711]
Journal?of?Cellular?and?Molecular?Medicine Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts [Pubmed: 30334324]
BMC?Veterinary Research Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome [Pubmed: 30404636]
International Journal of Basic and Clinical Endocrinology Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940]
MORPHOLOGY AND PATHOMORPHOLOGY Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment [Pubmed: 32152847]
JOURNAL OF DAIRY SCIENCE Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle [Pubmed: 32684470]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
Vet Microbiol Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection [34391195]
Antioxidants (Basel) Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574]
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459]
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