趨化素(CHEM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Chemerin (CHEM)
TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
- 編號SEA945Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.9pg/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測趨化素(CHEM)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的趨化素(CHEM)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-92 | 89 |
| EDTA plasma(n=5) | 91-99 | 94 |
| heparin plasma(n=5) | 86-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的趨化素(CHEM)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中趨化素(CHEM)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-104% | 97-105% | 80-102% | 83-95% |
| EDTA plasma(n=5) | 88-95% | 81-98% | 88-101% | 87-95% |
| heparin plasma(n=5) | 83-103% | 93-101% | 96-104% | 85-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將趨化素(CHEM)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的趨化素(CHEM)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的趨化素(CHEM)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的趨化素(CHEM)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA945Ra01 | 趨化素(CHEM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA945Ra01 | 趨化素(CHEM)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA945Ra91 | 趨化素(CHEM)多克隆抗體(辣根過氧化物酶標(biāo)記) | WB; IHC; ICC. |
| MAA945Ra24 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA945Ra23 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA945Ra22 | 趨化素(CHEM)單克隆抗體 | WB |
| MAA945Ra25 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA945Ra21 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA945Ra | 趨化素(CHEM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA945Ra | 趨化素(CHEM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Scandinavian Journal of Gastroenterology | Serum chemerin and vaspin in non-alcoholic fatty liver disease [PubMed: 20095887] |
| Gastroenterology & Hepatology | Chemerin, vaspin and insulin resistance in chronic hepatitis C [Wiley: source] |
| Placenta | Expression and regulation of chemerin during rat pregnancy [ScienceDirect: S0143400412000902] |
| Acta Pharmacologica Sinica | Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats [Nature: 201211] |
| RAED dergisi | Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci [Raeddergisi: Source] |
| Journal of Cellular and Molecular Medicine | Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients [Pubmed: 24702860] |
| Clinical Endocrinology | Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects [Pubmed: 24303851] |
| Oral Dis. | Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome [Pubmed:24106991] |
| Endocrine | Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719] |
| The Clinical Respiratory Journal | Relationship between plasma chemerin levels and disease severity in COPD patients [Pubmed:24865134] |
| Gynecol Endocrinol. | Elevated serum chemerin in Chinese women with hyperandrogenic PCOS [Pubmed:24927079] |
| Endocrine. | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. [Pubmed:25471281] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
| Dermatologica Sinica | Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris [Sciencedirect:S1027811714000470] |
| Dis Markers | Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study [PubMed: 26170530] |
| Med Sci Monit | Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population [PubMed: 26471865] |
| Endocrine | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats [PubMed: 25471281] |
| Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes [Pubmed:26721419] |
| Medicine (Baltimore). | Association?of?chemerin?levels?and?bone?mineral?density?in?Chinese?obese?postmenopausalwomen. [pubmed:27583869] |
| Oxidative?Medicine?and?Cellular?Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?… [Pubmed:29849871] |
| Current?Eye?Research | Chemerin is associated with diabetic retinopathy in type 2 diabetes [Pubmed:25848840] |
| Journal of?Oral?Rehabilitation | Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders [Pubmed:29331039] |
| International?Journal?of?Cancer | Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer [Pubmed: 30252132] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
| Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal [Doi: 10.1016/j.pan.2019.02.005] |
| Hypertens Pregnancy | Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin [Pubmed: 30922121] |
| Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal [Pubmed: 30833212] |
| Digestion | Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men [Pubmed: 31269491] |
| Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model [] | |
| Aging Medicine | Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus [] |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women [] |
| Int J Womens Health | Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women [Pubmed:35140527] |
