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表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

  • 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SEA560Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測表皮生長因子(EGF)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的表皮生長因子(EGF)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 87-105 95
EDTA plasma(n=5) 90-104 94
heparin plasma(n=5) 92-101 98

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的表皮生長因子(EGF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中表皮生長因子(EGF)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 94-101% 89-96% 91-101%
EDTA plasma(n=5) 78-96% 79-93% 91-98% 87-96%
heparin plasma(n=5) 99-105% 93-101% 80-104% 78-101%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將表皮生長因子(EGF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的表皮生長因子(EGF)與連接于固相載體上的抗體結合，然后加入生物素化的表皮生長因子(EGF)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的表皮生長因子(EGF)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

相關產品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
APA560Ra61 表皮生長因子(EGF)活性蛋白 Cell?culture;?Activity?Assays.
APA560Ra01 表皮生長因子(EGF)活性蛋白 Cell?culture;?Activity?Assays.
RPA560Ra01 表皮生長因子(EGF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA560Ra01 表皮生長因子(EGF)多克隆抗體 WB; IHC; ICC; IP.
MAA560Ra22 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
MAA560Ra23 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
MAA560Ra25 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
MAA560Ra27 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
MAA560Ra24 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
MAA560Ra21 表皮生長因子(EGF)單克隆抗體 WB; IHC; ICC; IP.
LAA560Ra72 表皮生長因子(EGF)單克隆抗體(生物素標記) WB; IHC; ICC.
SEA560Ra 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA560Ra 表皮生長因子(EGF)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA560Ra 表皮生長因子(EGF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PSA560Ra01 表皮生長因子(EGF)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA560Ra01 表皮生長因子(EGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195]
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
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Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
Aesthet Surg J Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732]
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Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377]
Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
Authorea Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy []
Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
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