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維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Vitamin D Receptor (VDR)

NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1,25-dihydroxyvitamin D3 receptor

  • 維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結(jié)果圖
  • SEA475Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測維生素D受體(VDR),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將維生素D受體(VDR)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的維生素D受體(VDR)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的維生素D受體(VDR)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的維生素D受體(VDR)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA475Ra01 維生素D受體(VDR)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA475Ra01 維生素D受體(VDR)多克隆抗體 WB; IHC; ICC; IP.
MAA475Ra21 維生素D受體(VDR)單克隆抗體 WB; IHC; ICC; IP.
SEA475Ra 維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA475Ra 維生素D受體(VDR)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
6 The vitamin D receptor: A therapeutic target for the treatment of breast cancer? [Cgi: Content]
endocrine-related cancer Vitamin D receptor as a target for breast cancer therapy. [pubmed:28213567]
Journal of Advanced?Research Effect?of?exercise?on?serum?vitamin D?and?tissue?vitamin Dreceptors?in?experimentally?induced?type 2 Diabetes Mellitus. [pubmed:27504197]
European Journal of Applied Physiology Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain [Pubmed:29143122]
BBA- Molecular Basis of Disease Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic?… [Pubmed: 30553018]
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus []
Journal of Steroid Biochemistry and Molecular Biology Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: a cross sectional study [Pubmed: 31255688]
Scientific Reports Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contacts [Pubmed: 31649297]
Research square Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression []
Betok Fish (Anabas testudineus) Oil Decreases Inflammatory Cytokine through Increasing Vitamin D Level in Rats-induced Systemic Lupus Erythematosus []
iium medical journal malaysia Positive Correlation between Monocyte-to-Lymphocyte Ratio and C-Reactive Protein in Vitamin D Deficient Preterm Infants with Respiratory Distress Syndrome []
Research Square Identification of SNP of VDR and VDBP gene and its Dysregulated pathway through VDR-VDBP interaction network analysis in Vitamin D deficient Infertile?… []
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