膠質(zhì)細(xì)胞系來(lái)源神經(jīng)營(yíng)養(yǎng)因子(GDNF)重組蛋白
Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF)
ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
- 編號(hào)RPA043Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位分泌
- 預(yù)測(cè)分子量16.9kDa
- 實(shí)際分子量19kDa(差異分析請(qǐng)參閱說(shuō)明書)
- 片段與標(biāo)簽Asp79~Leu217 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)8.9
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1051 ¥ 2628 ¥ 5256 ¥ 15768 ¥ 39420
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| ?Molecular Neurobiology | Efficient Generation of Functionally Active Spinal Cord Neurons from Spermatogonial Stem Cells [pubmed:27566610] |
| Chinese Medicial Journal | Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic Mice [PMC5717862] |
| The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg | Acupuncture Alters Pro-infl ammatory Cytokines in the Plasma of Maternally Separated Rat Pups [pubmed:28986807] |
| Heroin Addiction And Related Clinical Problems | GDNF serum levels are found to be lower in opioid-maintained patients [ISSN 2531-4122] |
| Journal of Environmental Research and Public Health | Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats [Pubmed: 30974849] |
| Frontiers in Aging Neuroscience | The Effects of Nordic Walking With Poles With an Integrated Resistance Shock Absorber on Cognitive Abilities and Cardiopulmonary Efficiency in?… [Pubmed: 33192480] |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
| Int J Biol Macromol | The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process [34119551] |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway [Pubmed:35366745] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
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